多聚半乳糖醛酸酶在成熟番茄果实中的免疫细胞化学定位。

Immunocytolocalization of polygalacturonase in ripening tomato fruit.

机构信息

Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907.

出版信息

Plant Physiol. 1989 May;90(1):17-20. doi: 10.1104/pp.90.1.17.

DOI:10.1104/pp.90.1.17

PMID:16666730

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1061667/

Abstract

Using tissue blotting and immunocytolocalization, we have investigated the appearance and accumulation of polygalacturonase (PG) during tomato (Lycopersicon esculentum Mill.) fruit ripening. Results show that PG first appears in the collumella region followed by sequential appearance in the exopericarp and endopericarp, respectively. Detectable levels of PG were not present in the locular material containing seeds. This result indicates that PG synthesis initiates at the central collumella region of tomato fruit during ripening.

摘要

利用组织印迹和免疫细胞化学定位技术，我们研究了番茄（Lycopersicon esculentum Mill.）果实成熟过程中多聚半乳糖醛酸酶（PG）的出现和积累。结果表明，PG 首先出现在果柄区，随后分别出现在外果皮和内果皮中。在含有种子的腔室材料中未检测到 PG。这一结果表明，PG 的合成在番茄果实成熟过程中从中央果柄区开始。