一种用于在缺氧气氛中筛选单个藻类菌落绝对光合速率的简单装置。
A simple apparatus for screening absolute photosynthetic rates of single algal colonies in an anoxic atmosphere.
机构信息
Chemical Technology Division, Oak Ridge National Laboratory , Oak Ridge, Tennessee 37831.
出版信息
Plant Physiol. 1989 May;90(1):246-50. doi: 10.1104/pp.90.1.246.
Photosynthetically generated O(2) was measured from single algal colonies in a He atmosphere, using an enhanced Hersch galvanic cell. The enhancement consisted of using ultrapure potassium hydroxide as the electrolyte and ultrapure lead as the anode. The galvanic cell was placed in a regulated helium-flow system containing a reaction cuvette with the colonies and an electrolysis cell for calibration. Colonies were individually irradiated using a He-Ne laser. Data collection and laser positioning for colony irradiation were microcomputer controlled. This assay system was capable of detecting O(2) production rates of 500 femtomoles per second with a signal to noise ratio of 2, a level of sensitivity that permitted the detection of photoevolved O(2) from single algal colonies. This capability provides, for the first time, an approach for quantitatively measuring the absolute rate of photosynthetic O(2) evolution from a single algal colony.
使用改进型赫歇尔电化池，从 He 气氛中的单个藻类菌落中测量光合作用产生的 O(2)。增强作用包括使用超纯氢氧化钾作为电解质和超纯铅作为阳极。电化池被放置在包含带有菌落的反应小室和用于校准的电解池的受控氦气流系统中。使用氦氖激光对菌落进行单独照射。数据收集和激光定位用于菌落照射由微机控制。该检测系统能够以信噪比为 2 检测到 500 飞摩尔/秒的 O(2)产生率，其灵敏度水平允许检测来自单个藻类菌落的光解 O(2)。这种能力首次提供了一种定量测量单个藻类菌落光合作用 O(2)释放绝对速率的方法。
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