Henstrand J M, Handa A K
Center for Plant Environmental Stress Physiology, Purdue University, West Lafayette, Indiana 47907.
Plant Physiol. 1989 Sep;91(1):157-62. doi: 10.1104/pp.91.1.157.
The contribution of wound-ethylene to wound-induced gene expression was investigated in unripe tomato pericarp using inhibitors of ethylene action. Wounded unripe tomato pericarp was treated with 2,5-norbornadiene or silver thiosulfate to inhibit specifically the induction of ethylene-dependent mRNA species. Poly(A)(+) RNAs isolated from these tissues after 12 hours of wounding were translated in vitro in a rabbit reticulocyte lysate system and [(35)S]methionine-labeled polypeptides were compared to unwounded controls after separation by one and two-dimensional polyacrylamide gel electrophoresis. Results show that mechanical wounding induces a dramatic shift in gene expression (over 50 mRNA species) but expression of less than 15% of these genes is affected by the treatment with ethylene action inhibitors. A selective decrease in mRNAs coding for a 37 kilodalton doublet and 75 kilodalton polypeptides is observed in 2,5-norbornadiene and silver thiosulfate treated wounded pericarp. Levels of hydroxyproline-rich glycoprotein mRNAs induced in wounded tissue were not influenced by inhibitors of ethylene action.
利用乙烯作用抑制剂，在未成熟番茄果皮中研究了创伤乙烯对创伤诱导基因表达的作用。用2,5-降冰片二烯或硫代硫酸银处理受伤的未成熟番茄果皮，以特异性抑制乙烯依赖性mRNA种类的诱导。受伤12小时后从这些组织中分离出的聚腺苷酸（Poly(A)(+)）RNA在兔网织红细胞裂解物系统中进行体外翻译，经一维和二维聚丙烯酰胺凝胶电泳分离后，将[³⁵S]甲硫氨酸标记的多肽与未受伤的对照进行比较。结果表明，机械创伤会导致基因表达发生显著变化（超过50种mRNA种类），但这些基因中不到15%的表达受乙烯作用抑制剂处理的影响。在经2,5-降冰片二烯和硫代硫酸银处理的受伤果皮中，观察到编码37千道尔顿双峰和75千道尔顿多肽的mRNA有选择性减少。受伤组织中诱导产生的富含羟脯氨酸糖蛋白mRNA的水平不受乙烯作用抑制剂的影响。
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