Department of Biochemistry, College of Agricultural and Life Science, University of Wisconsin-Madison, Madison, Wisconsin 53706.
Plant Physiol. 1989 Oct;91(2):451-4. doi: 10.1104/pp.91.2.451.
Pea, barley, and corn chromatin was isolated and subjected to limited digestion with DNasel. The preferentially degraded, small molecular weight fraction, presumably consisting of sequences in an active state of expression, was isolated and its 5-methylcytosine content determined. The DNasel-sensitive chromatin fraction from all three plant species investigated contained a markedly reduced level of DNA methylation compared to total DNA.
豌豆、大麦和玉米的染色质被分离出来,并使用 DNasel 进行有限消化。优先降解的、分子量较小的部分,可能由表达活跃状态的序列组成,被分离出来并测定其 5-甲基胞嘧啶含量。与总 DNA 相比,所有三种研究植物的 DNasel 敏感染色质部分的 DNA 甲基化水平明显降低。