Hoopes B C, McClure W R
Nucleic Acids Res. 1981 Oct 24;9(20):5493-504. doi: 10.1093/nar/9.20.5493.
We have examined the selectivity of the precipitation of DNA by spermine. We have found that the intra- and intermolecular condensation of DNA induced by spermine is highly selective even in the presence of added protein or triphosphates. We have also investigated the influence of buffer components on the threshold concentration of spermine required for DNA precipitation. Representative applications exploiting the selectivity of the precipitation reaction are also described.
我们研究了精胺对DNA沉淀的选择性。我们发现，即使在添加了蛋白质或三磷酸酯的情况下，精胺诱导的DNA分子内和分子间缩合仍具有高度选择性。我们还研究了缓冲液成分对DNA沉淀所需精胺阈值浓度的影响。文中还描述了利用沉淀反应选择性的代表性应用。
