Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1990 Jan;92(1):254-61. doi: 10.1104/pp.92.1.254.
Plastids were isolated from a plastome mutator-induced mutant (pm7) of Oenothera hookeri and were analyzed for various physiological and biochemical attributes. No photosynthetic electron transport activity was detected in the mutant plastids. This is consistent with previous ultrastructural analysis showing the absence of thylakoid membranes in the pm7 plastids and with the observation of aberrant processing and accumulation of chloroplast proteins in the mutant. In comparison to wild type, the mutant tissue lacks chlorophyll, and has significant differences in levels of four fatty acids. The analyses did not reveal any differences in carotenoid levels nor in the synthesis of several chloroplast lipids. The consequences of the altered composition of the chloroplast membrane are discussed in terms of their relation to the aberrant protein processing of the pm7 plastids. The pigment, fatty acid, and lipid measurements were also performed on two distinct nuclear genotypes (A/A and A/C) which differ in their compatibility with the plastid genome (type I) contained in these lines. In these cases, only chlorophyll concentrations differed significantly.
从月见草的一个质体突变体诱导突变体（pm7）中分离质体，并分析其各种生理和生化特性。在突变质体中未检测到光合电子传递活性。这与先前的超微结构分析结果一致，即 pm7 质体中没有类囊体膜，并且观察到突变体中叶绿体蛋白的异常加工和积累。与野生型相比，突变组织缺乏叶绿素，并且四种脂肪酸的水平有显著差异。分析未发现类胡萝卜素水平或几种叶绿体脂质的合成有任何差异。根据质体蛋白异常加工的关系，讨论了叶绿体膜组成改变的后果。还对两个不同的核基因型（A/A 和 A/C）进行了色素、脂肪酸和脂质的测量，这些基因型在与这些系中包含的质体基因组（I 型）的相容性上存在差异。在这些情况下，只有叶绿素浓度有显著差异。
