用 N，N-二甲基甲酰胺提取的叶绿素色素的测定公式。
Formulae for determination of chlorophyllous pigments extracted with n,n-dimethylformamide.
机构信息
Department of Botany, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.
出版信息
Plant Physiol. 1982 Jun;69(6):1376-81. doi: 10.1104/pp.69.6.1376.
Abstract
The extraction of chlorophylls in higher plant tissue using N,N-dimethylformamide expedites the process and enables the determination of small samples with low pigment level.Absorption spectra of Chl a, Chl b, and Pchl and of their acidified derivatives, the phaeophytins, were recorded. Conversion of Chl b to its corresponding acidified product occurs much more slowly than that of Chl a and Pchl. When acidified, Pchl differs from Chl a and Chl b by the disappearance of the red band in the absorption spectrum. Specific extinction coefficients were determined and formulae for quantitative determination of pigments concentrations were developed. When concentrations of pigments are low, as in etiolated plant material, the absorption spectra of the chlorophylls can be distorted due to the presence of other substances simultaneously extracted; formulae for pigment determination under such circumstances were also derived.
摘要
用 N,N-二甲基甲酰胺提取高等植物组织中的叶绿素可加速该过程，并能对低色素含量的小样本进行测定。记录了 Chl a、Chl b 和 Pchl 及其酸化衍生物（脱镁叶绿素）的吸收光谱。Chl b 转化为其相应的酸化产物的速度比 Chl a 和 Pchl 慢得多。酸化时，Pchl 在吸收光谱中红色波段的消失与 Chl a 和 Chl b 不同。确定了特定的消光系数，并制定了定量测定色素浓度的公式。当色素浓度较低时，例如在黄化植物材料中，由于同时提取的其他物质的存在，叶绿素的吸收光谱可能会发生扭曲；还推导了在此情况下用于色素测定的公式。
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本文引用的文献
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Plant Physiol. 1980 Mar;65(3):478-9. doi: 10.1104/pp.65.3.478.
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