Department of Botany, University of British Columbia, Vancouver, B.C., Canada V6T 2B1.
Plant Physiol. 1990 Aug;93(4):1426-32. doi: 10.1104/pp.93.4.1426.
(13)NO(3) (-) was used to investigate patterns of NO(3) (-) influx into roots of barley plants (Hordeum vulgare L. cv Klondike) previously grown with (;induced') or without (;uninduced') a source of external NO(3) (-) (NO(3) (-)). In both induced and uninduced plants, (13)NO(3) (-) influx was biphasic in the range from 0.005 to 50 moles per cubic meter NO(3) (-). In the low concentration range (<1 mole per cubic meter for induced plants and <0.3 mole per cubic meter for uninduced plants), influx was saturable and V(max) and K(m) values for influx either increased or decreased according to NO(3) (-) pretreatment. By contrast, (13)NO(3) (-) influx in the high concentration range revealed a strictly linear concentration dependence. These fluxes appeared to be mediated by a constitutive, rather than an inducible, transport system.
(13)NO3(-)被用来研究先前在有(诱导)或没有(未诱导)外部 NO3(-)源[NO3(-)](0)生长的大麦植物(Hordeum vulgare L. cv Klondike)根部中 NO3(-)流入的模式。在诱导和未诱导的植物中,(13)NO3(-)的流入在 0.005 至 50 毫摩尔/立方米[NO3(-)](0)的范围内呈双相。在低浓度范围内(诱导植物 <1 毫摩尔/立方米,未诱导植物 <0.3 毫摩尔/立方米),流入是可饱和的,流入的 V(max)和 K(m)值根据 NO3(-)预处理而增加或减少。相比之下,(13)NO3(-)在高浓度范围内的流入显示出严格的线性浓度依赖性。这些通量似乎是由组成型而不是诱导型运输系统介导的。