体内应激诱导的1-氨基环丙烷羧酸合酶的失活与体外底物依赖性失活不同。
Inactivation of stress induced 1-aminocyclopropane carboxylate synthase in vivo differs from substrate-dependent inactivation in vitro.
作者信息
Spanu P, Felix G, Boller T
机构信息
Botanisches Institut der Universität Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland.
出版信息
Plant Physiol. 1990 Aug;93(4):1482-5. doi: 10.1104/pp.93.4.1482.
Abstract
The activity of 1-aminocyclopropane carboxylate (ACC) synthase increased rapidly in tomato (Lycopersicon esculentum Mill.) leaf discs after vacuum infiltration, reached a maximum after about 30 minutes, and subsequently decayed with an apparent half-life of about 20 minutes. Aminoethoxyvinylglycine, a known inhibitor of ACC synthase, did not alter the apparent turnover of ACC synthase in vivo although it efficiently blocked inactivation of the enzyme by its substrate S-adenosylmethionine in vitro. Similar results were obtained, using a novel assay with permeabilized cells, for ACC synthase in tomato cell cultures treated with a fungal elicitor. The results indicate that inactivation of ACC synthase in vivo differs from substrate-dependent inactivation in vitro.
摘要
番茄（Lycopersicon esculentum Mill.）叶片圆片在真空渗入后，1-氨基环丙烷羧酸（ACC）合酶的活性迅速增加，约30分钟后达到最大值，随后以约20分钟的表观半衰期衰减。氨基乙氧基乙烯基甘氨酸是一种已知的ACC合酶抑制剂，虽然它在体外能有效阻断该酶被其底物S-腺苷甲硫氨酸的失活，但在体内并未改变ACC合酶的表观周转。使用一种针对透化细胞的新检测方法，对用真菌激发子处理的番茄细胞培养物中的ACC合酶进行检测，也得到了类似结果。这些结果表明，ACC合酶在体内的失活与体外的底物依赖性失活不同。
相似文献
1
Inactivation of stress induced 1-aminocyclopropane carboxylate synthase in vivo differs from substrate-dependent inactivation in vitro.体内应激诱导的1-氨基环丙烷羧酸合酶的失活与体外底物依赖性失活不同。
Plant Physiol. 1990 Aug;93(4):1482-5. doi: 10.1104/pp.93.4.1482.
2
Turnover of 1-aminocyclopropane-1-carboxylic Acid synthase protein in wounded tomato fruit tissue.1-氨基环丙烷-1-羧酸合酶蛋白在受伤番茄果实组织中的周转。
Plant Physiol. 1992 Nov;100(3):1126-31. doi: 10.1104/pp.100.3.1126.
3
The Apparent Turnover of 1-Aminocyclopropane-1-Carboxylate Synthase in Tomato Cells Is Regulated by Protein Phosphorylation and Dephosphorylation.番茄细胞中1-氨基环丙烷-1-羧酸合成酶的表观周转率受蛋白质磷酸化和去磷酸化调控。
Plant Physiol. 1994 Oct;106(2):529-535. doi: 10.1104/pp.106.2.529.
4
Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits.S-腺苷-L-甲硫氨酸对从番茄果实中分离出的1-氨基环丙烷-1-羧酸合酶的失活作用及标记特异性
Arch Biochem Biophys. 1989 May 15;271(1):107-12. doi: 10.1016/0003-9861(89)90260-9.
5
Wound ethylene and 1-aminocyclopropane-1-carboxylate synthase in ripening tomato fruit.伤口乙烯和 1-氨基环丙烷-1-羧酸合酶在成熟番茄果实中。
Planta. 1981 May;151(5):476-81. doi: 10.1007/BF00386542.
6
S-adenosylmethionine-dependent inactivation and radiolabeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits.依赖 S-腺苷甲硫氨酸的失活和放射性标记番茄果实中分离的 1-氨基环丙烷-1-羧酸合酶。
Plant Physiol. 1988 Sep;88(1):109-14. doi: 10.1104/pp.88.1.109.
7
Elicitor-induced ethylene biosynthesis in tomato cells: characterization and use as a bioassay for elicitor action.激发子诱导番茄细胞中乙烯生物合成：特性及作为激发子活性生物测定方法的应用
Plant Physiol. 1991 Sep;97(1):19-25. doi: 10.1104/pp.97.1.19.
8
A comparison of 1-aminocyclopropane-1-carboxylate synthase in vitro translation product and in-vivo-labeled protein in ripening tomatoes.成熟番茄中 1-氨基环丙烷-1-羧酸合成酶的体外翻译产物与体内标记蛋白的比较。
Planta. 1990 Nov;182(4):635-8. doi: 10.1007/BF02341042.
9
A functional tomato ACC synthase expressed in Escherichia coli demonstrates suicidal inactivation by its substrate S-adenosylmethionine.一种在大肠杆菌中表达的功能性番茄ACC合成酶表现出被其底物S-腺苷甲硫氨酸自杀性失活。
FEBS Lett. 1992 Jul 20;306(2-3):103-7. doi: 10.1016/0014-5793(92)80978-p.
10
Properties and Partial Purification of 1-Aminocyclopropane-1-carboxylate Synthase.1-氨基环丙烷-1-羧酸合酶的性质与部分纯化
Plant Physiol. 1983 May;72(1):139-45. doi: 10.1104/pp.72.1.139.
引用本文的文献
1
Elicitor-induced ethylene biosynthesis in tomato cells: characterization and use as a bioassay for elicitor action.激发子诱导番茄细胞中乙烯生物合成：特性及作为激发子活性生物测定方法的应用
Plant Physiol. 1991 Sep;97(1):19-25. doi: 10.1104/pp.97.1.19.
2
Turnover of 1-aminocyclopropane-1-carboxylic Acid synthase protein in wounded tomato fruit tissue.1-氨基环丙烷-1-羧酸合酶蛋白在受伤番茄果实组织中的周转。
Plant Physiol. 1992 Nov;100(3):1126-31. doi: 10.1104/pp.100.3.1126.
3
The eto1, eto2, and eto3 mutations and cytokinin treatment increase ethylene biosynthesis in Arabidopsis by increasing the stability of ACS protein.eto1、eto2和eto3突变以及细胞分裂素处理通过增加ACS蛋白的稳定性来提高拟南芥中的乙烯生物合成。
Plant Cell. 2003 Feb;15(2):545-59. doi: 10.1105/tpc.006882.
4
Regulation of Protein Degradation.蛋白质降解的调控
Plant Cell. 1995 Jul;7(7):845-857. doi: 10.1105/tpc.7.7.845.
5
The Apparent Turnover of 1-Aminocyclopropane-1-Carboxylate Synthase in Tomato Cells Is Regulated by Protein Phosphorylation and Dephosphorylation.番茄细胞中1-氨基环丙烷-1-羧酸合成酶的表观周转率受蛋白质磷酸化和去磷酸化调控。
Plant Physiol. 1994 Oct;106(2):529-535. doi: 10.1104/pp.106.2.529.
6
The protein phosphatase inhibitor calyculin A mimics elicitor action in plant cells and induces rapid hyperphosphorylation of specific proteins as revealed by pulse labeling with [33P]phosphate.蛋白磷酸酶抑制剂花萼海绵诱癌素A在植物细胞中模拟激发子作用，并通过用[33P]磷酸盐脉冲标记揭示其诱导特定蛋白质的快速超磷酸化。
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):952-6. doi: 10.1073/pnas.91.3.952.
7
Sensing of osmotic pressure changes in tomato cells.番茄细胞中渗透压变化的感知
Plant Physiol. 2000 Nov;124(3):1169-80. doi: 10.1104/pp.124.3.1169.
8
Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively.拟南芥乙烯生物合成基因ACS5中的隐性和显性突变分别导致对细胞分裂素不敏感和乙烯过量产生。
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4766-71. doi: 10.1073/pnas.95.8.4766.
9
Differential induction of seven 1-aminocyclopropane-1-carboxylate synthase genes by elicitor in suspension cultures of tomato (Lycopersicon esculentum).激发子对番茄（Lycopersicon esculentum）悬浮培养物中七个1-氨基环丙烷-1-羧酸合酶基因的差异诱导作用
Plant Mol Biol. 1997 May;34(2):275-86. doi: 10.1023/a:1005800511372.
10
Analysis and cloning of the ethylene-forming enzyme from tomato by functional expression of its mRNA in Xenopus laevis oocytes.通过在非洲爪蟾卵母细胞中功能性表达其mRNA对番茄乙烯形成酶进行分析和克隆。
EMBO J. 1991 Aug;10(8):2007-13. doi: 10.1002/j.1460-2075.1991.tb07730.x.
本文引用的文献
1
S-adenosylmethionine-dependent inactivation and radiolabeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits.依赖 S-腺苷甲硫氨酸的失活和放射性标记番茄果实中分离的 1-氨基环丙烷-1-羧酸合酶。
Plant Physiol. 1988 Sep;88(1):109-14. doi: 10.1104/pp.88.1.109.
2
Properties and Partial Purification of 1-Aminocyclopropane-1-carboxylate Synthase.1-氨基环丙烷-1-羧酸合酶的性质与部分纯化
Plant Physiol. 1983 May;72(1):139-45. doi: 10.1104/pp.72.1.139.
3
Mechanism of inhibition of spinach beta-cystathionase by rhizobitoxine.根瘤毒素对菠菜β-胱硫醚酶的抑制机制。
Biochim Biophys Acta. 1971 Mar 10;227(3):671-84. doi: 10.1016/0005-2744(71)90016-7.
4
Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of 1-aminocyclopropane-1-carboxylate synthase isolated from tomato fruits.S-腺苷-L-甲硫氨酸对从番茄果实中分离出的1-氨基环丙烷-1-羧酸合酶的失活作用及标记特异性
Arch Biochem Biophys. 1989 May 15;271(1):107-12. doi: 10.1016/0003-9861(89)90260-9.
5
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
6
A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid.一种用于检测1-氨基环丙烷-1-羧酸的简单灵敏的测定方法。
Anal Biochem. 1979 Nov 15;100(1):140-5. doi: 10.1016/0003-2697(79)90123-4.
Zotero 插件