拟南芥乙烯生物合成基因ACS5中的隐性和显性突变分别导致对细胞分裂素不敏感和乙烯过量产生。
Recessive and dominant mutations in the ethylene biosynthetic gene ACS5 of Arabidopsis confer cytokinin insensitivity and ethylene overproduction, respectively.
作者信息
Vogel J P, Woeste K E, Theologis A, Kieber J J
机构信息
Department of Biological Sciences, Laboratory for Molecular Biology, University of Illinois, Chicago, IL 60607, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4766-71. doi: 10.1073/pnas.95.8.4766.
Abstract
We identified a set of cytokinin-insensitive mutants by using a screen based on the ethylene-mediated triple response observed after treatment with low levels of cytokinins. One group of these mutants disrupts ACS5, a member of the Arabidopsis gene family that encodes 1-aminocyclopropane-1-carboxylate synthase, the first enzyme in ethylene biosynthesis. The ACS5 isoform is mainly responsible for the sustained rise in ethylene biosynthesis observed in response to low levels of cytokinin and appears to be regulated primarily by a posttranscriptional mechanism. Furthermore, the dominant ethylene-overproducing mutant eto2 was found to be the result of an alteration of the carboxy terminus of ACS5, suggesting that this domain acts as a negative regulator of ACS5 function.
摘要
我们通过基于低水平细胞分裂素处理后观察到的乙烯介导的三重反应的筛选，鉴定出一组细胞分裂素不敏感突变体。其中一组突变体破坏了ACS5，它是拟南芥基因家族的一个成员，该家族编码1-氨基环丙烷-1-羧酸合酶，这是乙烯生物合成中的第一种酶。ACS5同工型主要负责响应低水平细胞分裂素时观察到的乙烯生物合成的持续增加，并且似乎主要受转录后机制调控。此外，发现显性乙烯过量产生突变体eto2是ACS5羧基末端改变的结果，这表明该结构域作为ACS5功能的负调节因子。
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