Departamento de Bioquimica, Facultad de Quimica, U.N.A.M. México, D.F. 04510.
Plant Physiol. 1990 Nov;94(3):1270-5. doi: 10.1104/pp.94.3.1270.
The effect of auxin on ribosomal protein phosphorylation of germinating maize (Zea mays) tissues was investigated. Two-dimensional gel electrophoresis and autoradiography of [(32)P] ribosomal protein patterns for natural and synthetic auxin-treated tissues were performed. Both the rate of (32)P incorporation and the electrophoretic patterns were dependent on (32)P pulse length, suggesting that active protein phosphorylation-dephosphorylation occurred in small and large subunit proteins, in control as well as in auxin-treated tissues. The effect of ribosomal protein phosphorylation on in vitro translation was tested. Measurements of poly(U) translation rates as a function of ribosome concentration provided apparent K(m) values significantly different for auxin-treated and nontreated tissues. These findings suggest that auxin might exert some kind of translational control by regulating the phosphorylated status of ribosomal proteins.
研究了生长素对萌发玉米（Zea mays）组织核糖体蛋白磷酸化的影响。对天然和合成生长素处理组织的[(32)P]核糖体蛋白图谱进行二维凝胶电泳和放射自显影。(32)P 掺入率和电泳图谱均依赖于(32)P 脉冲长度，表明在对照和生长素处理的组织中，小亚基和大亚基蛋白均发生了活性蛋白磷酸化-去磷酸化。还测试了核糖体蛋白磷酸化对体外翻译的影响。作为核糖体浓度函数的多（U）翻译速率的测量为生长素处理和未处理组织提供了明显不同的表观 K(m)值。这些发现表明，生长素可能通过调节核糖体蛋白的磷酸化状态来发挥某种翻译控制作用。
