Huttly A K, Phillips A L
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, UK.
Plant Mol Biol. 1995 Mar;27(5):1043-52. doi: 10.1007/BF00037031.
cDNA fragments from ten different protein kinases expressed in Avena sativa aleurone cells were amplified from mRNA by RT-PCR with degenerate primers. These could be classified into five groups: Aspk1-3 showed homology to the Snf1-related protein kinases, Aspk4-5 to a wheat ABA up-regulated protein kinase, Aspk6-8 to the Ca-dependent, calmodulin-independent protein kinase family, Aspk9 encoded a MAP kinase and Aspk10 was closely related to a novel Arabidopsis ribosomal protein kinase. GA caused a rapid increase in transcripts hybridising to Aspk10, while inhibiting the dramatic accumulation of transcripts hybridising to Aspk9 that occurred in the absence of GA.
利用简并引物通过逆转录聚合酶链反应（RT-PCR）从燕麦糊粉层细胞中表达的10种不同蛋白激酶的信使核糖核酸（mRNA）扩增出互补脱氧核糖核酸（cDNA）片段。这些片段可分为五组：Aspk1 - 3与Snf1相关蛋白激酶具有同源性，Aspk4 - 5与小麦脱落酸上调蛋白激酶具有同源性，Aspk6 - 8与钙依赖、钙调蛋白非依赖蛋白激酶家族具有同源性，Aspk9编码一种促分裂原活化蛋白激酶（MAP激酶），Aspk10与一种新的拟南芥核糖体蛋白激酶密切相关。赤霉素（GA）导致与Aspk10杂交的转录本迅速增加，同时抑制了在无GA情况下与Aspk9杂交的转录本的显著积累。
