克隆和失活对集胞藻 PCC6803 无机碳运输至关重要的基因。
Cloning and Inactivation of a Gene Essential to Inorganic Carbon Transport of Synechocystis PCC6803.
机构信息
Solar Energy Research Group, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama 351-01, Japan.
出版信息
Plant Physiol. 1991 May;96(1):280-4. doi: 10.1104/pp.96.1.280.
Abstract
A clone (HP-1) which transforms the high CO(2)-requiring mutant (RKb) of Synechocystis PCC6803 defective in inorganic carbon transport to the wild-type (WT) phenotype was isolated from a WT genomic library. The clone contained a 5.4 kilobase-pair DNA insert. Complementation tests with subclones derived from HP-1 allowed the mutation in RKb to be located within 141 base-pair nucleotides. Sequencing of nucleotides in this region revealed an open reading frame encoding a hydrophobic protein consists of 80 amino acids. A defined mutant (M9) constructed by inactivating this putative inorganic carbon transport gene, designated ictA, was unable to transport CO(2) and HCO(3) (-) into the intracellular inorganic carbon pool. Cloning and sequence analysis of the respective RKb gene revealed a base substitution which generates a stop codon in the middle of ictA.
摘要
从野生型（WT）基因组文库中分离到一个克隆（HP-1），该克隆能够将对二氧化碳要求较高的突变体（RKb）转化为无机碳转运缺陷的集胞藻 PCC6803 的野生型（WT）表型。该克隆含有一个 5.4 千碱基对的 DNA 插入片段。用 HP-1 衍生的亚克隆进行互补测试，允许将 RKb 中的突变定位在 141 个碱基对核苷酸内。对该区域核苷酸的测序揭示了一个开放阅读框，编码一个由 80 个氨基酸组成的疏水性蛋白。通过使这个假定的无机碳转运基因失活构建的一个明确的突变体（M9），命名为 ictA，无法将 CO（2）和 HCO（3）（-）转运到细胞内的无机碳池中。对相应的 RKb 基因的克隆和序列分析表明，碱基取代在 ictA 中间产生了一个终止密码子。
相似文献
1
Cloning and Inactivation of a Gene Essential to Inorganic Carbon Transport of Synechocystis PCC6803.克隆和失活对集胞藻 PCC6803 无机碳运输至关重要的基因。
Plant Physiol. 1991 May;96(1):280-4. doi: 10.1104/pp.96.1.280.
2
A gene homologous to the subunit-2 gene of NADH dehydrogenase is essential to inorganic carbon transport of Synechocystis PCC6803.与NADH脱氢酶亚基2基因同源的一个基因对集胞藻PCC6803的无机碳转运至关重要。
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4275-9. doi: 10.1073/pnas.88.10.4275.
3
A gene (ccmA) required for carboxysome formation in the cyanobacterium Synechocystis sp. strain PCC6803.集胞藻6803（Synechocystis sp. strain PCC6803）中羧酶体形成所需的一个基因（ccmA）。
J Bacteriol. 1994 Apr;176(8):2374-8. doi: 10.1128/jb.176.8.2374-2378.1994.
4
Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803.鉴定和表征对集胞藻 PCC6803 无机碳运输至关重要的ictA/ndhL 基因产物。
Plant Physiol. 1992 Aug;99(4):1604-8. doi: 10.1104/pp.99.4.1604.
5
Isolation and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization.分离和鉴定集胞藻 PCC6803 利用无机碳所需的 ccmM 基因。
Photosynth Res. 1994 Feb;39(2):183-90. doi: 10.1007/BF00029385.
6
Mutants of Synechocystis PCC6803 Defective in Inorganic Carbon Transport.集胞藻PCC6803中无机碳转运缺陷型突变体。
Plant Physiol. 1990 Oct;94(2):760-5. doi: 10.1104/pp.94.2.760.
7
cemA homologue essential to CO2 transport in the cyanobacterium Synechocystis PCC6803.集胞藻PCC6803中对二氧化碳运输至关重要的cemA同源物。
Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4006-10. doi: 10.1073/pnas.93.9.4006.
8
Uptake of inorganic carbon in the cyanobacterium Synechocystis PCC6803: physiological and genetic evidence for a high-affinity uptake system.集胞藻PCC6803中无机碳的摄取：高亲和力摄取系统的生理学和遗传学证据
Mol Microbiol. 1995 Nov;18(3):559-68. doi: 10.1111/j.1365-2958.1995.mmi_18030559.x.
9
Characterization of a mutant lacking carboxysomal carbonic anhydrase from the cyanobacterium Synechocystis PCC6803.来自集胞藻PCC6803的缺乏羧酶体碳酸酐酶的突变体的特性分析。
Planta. 2002 Jan;214(3):456-67. doi: 10.1007/s004250100638.
10
Synechocystis sp. PCC6803 possesses a two-component polyhydroxyalkanoic acid synthase similar to that of anoxygenic purple sulfur bacteria.聚球藻属嗜盐光合细菌PCC6803拥有一种双组分聚羟基链烷酸合酶，类似于不产氧紫色硫细菌的该种酶。
Arch Microbiol. 1998 Sep;170(3):162-70. doi: 10.1007/s002030050629.
引用本文的文献
1
Cyanobacterial NDH-1 Complexes.蓝藻NDH-1复合体
Front Microbiol. 2022 Jul 1;13:933160. doi: 10.3389/fmicb.2022.933160. eCollection 2022.
2
The NDH-1L-PSI Supercomplex Is Important for Efficient Cyclic Electron Transport in Cyanobacteria.NDH-1L-PSI超复合体对蓝藻中高效循环电子传递很重要。
Plant Physiol. 2016 Nov;172(3):1451-1464. doi: 10.1104/pp.16.00585. Epub 2016 Sep 12.
3
Network analysis of transcriptomics expands regulatory landscapes in Synechococcus sp. PCC 7002.转录组学的网络分析拓展了聚球藻属PCC 7002中的调控格局。
Nucleic Acids Res. 2016 Oct 14;44(18):8810-8825. doi: 10.1093/nar/gkw737. Epub 2016 Aug 27.
4
Effects of inorganic carbon accumulation on photosynthetic oxygen reduction and cyclic electron flow in the cyanobacterium Synechococcus PCC7942.无机碳积累对蓝细菌集胞藻 PCC7942 光合放氧还原和环式电子流的影响。
Photosynth Res. 1993 Sep;37(3):177-91. doi: 10.1007/BF00032822.
5
Isolation and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization.分离和鉴定集胞藻 PCC6803 利用无机碳所需的 ccmM 基因。
Photosynth Res. 1994 Feb;39(2):183-90. doi: 10.1007/BF00029385.
6
Cyanobacterial NADPH dehydrogenase complexes.蓝藻NADPH脱氢酶复合体
Photosynth Res. 2007 Jul-Sep;93(1-3):69-77. doi: 10.1007/s11120-006-9128-y. Epub 2007 Feb 6.
7
Identification and Characterization of the ictA/ndhL Gene Product Essential to Inorganic Carbon Transport of Synechocystis PCC6803.鉴定和表征对集胞藻 PCC6803 无机碳运输至关重要的ictA/ndhL 基因产物。
Plant Physiol. 1992 Aug;99(4):1604-8. doi: 10.1104/pp.99.4.1604.
8
Inorganic carbon acquisition systems in cyanobacteria.蓝细菌中的无机碳获取系统。
Photosynth Res. 2003;77(2-3):105-15. doi: 10.1023/A:1025865500026.
9
Towards functional proteomics of membrane protein complexes in Synechocystis sp. PCC 6803.迈向集胞藻6803中膜蛋白复合物的功能蛋白质组学研究
Plant Physiol. 2004 Jan;134(1):470-81. doi: 10.1104/pp.103.032326.
10
Insertional mutants of Chlamydomonas reinhardtii that require elevated CO(2) for survival.莱茵衣藻的插入突变体，其生存需要高浓度二氧化碳。
Plant Physiol. 2001 Oct;127(2):607-14.
本文引用的文献
1
Mutants of Synechocystis PCC6803 Defective in Inorganic Carbon Transport.集胞藻PCC6803中无机碳转运缺陷型突变体。
Plant Physiol. 1990 Oct;94(2):760-5. doi: 10.1104/pp.94.2.760.
2
Ethoxyzolamide Inhibition of CO(2) Uptake in the Cyanobacterium Synechococcus PCC7942 without Apparent Inhibition of Internal Carbonic Anhydrase Activity.乙氧唑胺对蓝藻聚球藻Synechococcus PCC7942中CO₂摄取的抑制作用，而对其内部碳酸酐酶活性无明显抑制。
Plant Physiol. 1989 Jan;89(1):37-43. doi: 10.1104/pp.89.1.37.
3
Characterization of the na-requirement in cyanobacterial photosynthesis.蓝藻光合作用中钠需求的特征描述。
Plant Physiol. 1988 Nov;88(3):757-63. doi: 10.1104/pp.88.3.757.
4
Simultaneous Transport of CO(2) and HCO(3) by the Cyanobacterium Synechococcus UTEX 625.蓝藻 Synechococcus UTEX 625 同时运输 CO(2)和 HCO(3)。
Plant Physiol. 1988 Jul;87(3):551-4. doi: 10.1104/pp.87.3.551.
5
Energization and activation of inorganic carbon uptake by light in cyanobacteria.蓝细菌中无机碳摄取的光激发和激活。
Plant Physiol. 1987 Jun;84(2):210-3. doi: 10.1104/pp.84.2.210.
6
The Stoichiometry between CO(2) and H Fluxes Involved in the Transport of Inorganic Carbon in Cyanobacteria.蓝细菌中无机碳转运过程中CO₂与H通量之间的化学计量关系。
Plant Physiol. 1987 Apr;83(4):888-91. doi: 10.1104/pp.83.4.888.
7
Nature of the Inorganic Carbon Species Actively Taken Up by the Cyanobacterium Anabaena variabilis.可变鱼腥藻主动吸收的无机碳物种的性质。
Plant Physiol. 1984 Nov;76(3):599-602. doi: 10.1104/pp.76.3.599.
8
Internal Inorganic Carbon Pool of Chlamydomonas reinhardtii: EVIDENCE FOR A CARBON DIOXIDE-CONCENTRATING MECHANISM.莱茵衣藻的体内无机碳库：二氧化碳浓缩机制的证据。
Plant Physiol. 1980 Sep;66(3):407-13. doi: 10.1104/pp.66.3.407.
9
A simple method for displaying the hydropathic character of a protein.一种展示蛋白质亲水性特征的简单方法。
J Mol Biol. 1982 May 5;157(1):105-32. doi: 10.1016/0022-2836(82)90515-0.
10
Active transport and accumulation of bicarbonate by a unicellular cyanobacterium.单细胞蓝细菌对碳酸氢盐的主动运输与积累
J Bacteriol. 1980 Sep;143(3):1253-9. doi: 10.1128/jb.143.3.1253-1259.1980.
Zotero 插件