创伤烟草叶片会导致内源吲哚-3-乙酸的减少。
Wounding Nicotiana tabacum Leaves Causes a Decline in Endogenous Indole-3-Acetic Acid.
机构信息
Department of Biochemistry and Biophysics, Iowa State University, Ames, Iowa 50011.
出版信息
Plant Physiol. 1991 Jul;96(3):802-5. doi: 10.1104/pp.96.3.802.
Abstract
We have previously observed that auxin can act as a repressor of the wound-inducible activation of a chimeric potato proteinase inhibitor II-CAT chimeric gene (pin2-CAT) in transgenic tobacco (Nicotiana tobacum) callus and in whole plants. Therefore, this study was designed to examine endogenous levels of indole-3-acetic acid (IAA) in plant tissues both before and after wounding. Endogenous IAA was measured in whole plant tissues by gas chromatography-mass spectrometry using an isotope dilution technique. (13)C-Labeled IAA was used as an internal standard. The endogenous levels of IAA declined two- to threefold within 6 hours after a wound. The kinetics of auxin decline are consistent with the kinetics of activation of the pin2-CAT construction in the foliage of transgenic tobacco.
摘要
我们之前已经观察到，在转基因烟草（Nicotiana tobacum）愈伤组织和整株植物中，生长素可以作为一种抑制剂，抑制嵌合马铃薯蛋白酶抑制剂 II-CAT 嵌合基因（pin2-CAT）的诱导型激活。因此，本研究旨在检测植物组织中内源性吲哚-3-乙酸（IAA）的水平，包括在受伤前后。通过使用同位素稀释技术的气相色谱-质谱联用仪，在整株植物组织中测量内源性 IAA。（13）C 标记的 IAA 被用作内标。受伤后 6 小时内，IAA 的内源性水平下降了两到三倍。生长素下降的动力学与转基因烟草叶片中 pin2-CAT 构建体的激活动力学一致。
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