Weiss C, Bevan M
Cambridge Laboratory, Colney Lane, Norwich NR4 7UH England.
Plant Physiol. 1991 Jul;96(3):943-51. doi: 10.1104/pp.96.3.943.
The transcriptional regulation of a win2-beta-glucuronidase gene fusion in transgenic potato (Solanum tuberosum) plants by wounding and ethylene has been analyzed. In common with other genes that are expressed in response to mechanical or chemical stress, win2 is transcribed at the site of injury and also in distant undamaged parts of the wounded plant. Similar kinetics of induction and patterns of transcription were observed in response to mechanical, wounding, elicitor, or arachidonic acid application. Experiments involving the use of chemicals that inhibited ethylene action, and those that increased ethylene production, showed that local induction of win2 transcription did not have an absolute requirement for ethylene, but ethylene was necessary for high levels of expression. In contrast, systemic expression of win2 required both a putative wound signal and ethylene. Ethylene alone had no direct effect on win2 gene expression in the absence of wounding.
已对转基因马铃薯（Solanum tuberosum）植株中由创伤和乙烯介导的win2-β-葡萄糖醛酸酶基因融合体的转录调控进行了分析。与其他响应机械或化学胁迫而表达的基因一样，win2在损伤部位以及受伤植株未受损的远端部位均有转录。在响应机械损伤、创伤、激发子或花生四烯酸处理时，观察到了相似的诱导动力学和转录模式。使用抑制乙烯作用的化学物质以及增加乙烯产生的化学物质的实验表明，win2转录的局部诱导对乙烯并非绝对必需，但乙烯对于高水平表达是必需的。相比之下，win2的系统性表达既需要假定的创伤信号，也需要乙烯。在没有创伤的情况下，单独的乙烯对win2基因表达没有直接影响。
