The Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
Plant Physiol. 1973 Jun;51(6):1033-6. doi: 10.1104/pp.51.6.1033.
Ethylene enhanced the activity of phenylalanine ammonialyase in carrot (Daucus carota L., var. "Nauty") root tissue. Slight increase in enzyme activity was exhibited by root discs incubated in ethylene-free air. It was probably due to the ethylene formed within the sliced tissue. Addition of ethylene to the air stream increased phenylalanine ammonia-lyase activity and the total protein content of the discs until maximum activity was reached after 36 to 48 hours of incubation. The continuous presence of ethylene was required to maintain high level of activity. Ethylene, at a concentration of 10 microliter per liter induced higher activity than at lower or higher concentrations. CO(2) partially inhibited the ethylene-induced activity. Cycloheximide or actinomycin D effectively inhibited the ethylene-induced activity in discs that had not previously been exposed to ethylene. The results appear to support the hypothesis that the mode of action of ethylene may involve both de novo synthesis of the enzyme protein and protection or regulation of activity of the induced enzyme.
乙烯增强了胡萝卜（Daucus carota L.，var. “Nauty”）根组织中苯丙氨酸解氨酶的活性。在无乙烯的空气中孵育的根圆盘显示出轻微的酶活性增加。这可能是由于切片组织内形成的乙烯所致。向气流中添加乙烯会增加苯丙氨酸氨解酶的活性和圆盘的总蛋白含量，直到孵育 36 至 48 小时后达到最大活性。需要持续存在乙烯才能维持高活性。在 10 微升/升的浓度下，乙烯诱导的活性高于较低或较高浓度。CO（2）部分抑制了乙烯诱导的活性。环己酰亚胺或放线菌素 D 可有效抑制先前未暴露于乙烯的圆盘中的乙烯诱导的活性。结果似乎支持这样一种假设，即乙烯的作用模式可能既涉及酶蛋白的从头合成，又涉及诱导酶的活性的保护或调节。
