Robertson G R, Scott N A, Miller J M, Sabine M, Zheng M, Bell C W, Whalley J M
School of Biological Sciences, Macquarie University, Sydney, Australia.
DNA Seq. 1991;1(4):241-9. doi: 10.3109/10425179109020779.
A gene in equine herpesvirus 1 (EHV-1, equine abortion virus) homologous to the glycoprotein H gene of herpes simplex virus (HSV) was identified and characterised by its nucleotide and derived amino acid sequence. The EHV-1 gH gene is located at 0.47-0.49 map units and contains an open reading frame capable of specifying a polypeptide of 848 amino acids, including N- and C-terminal hydrophobic domains consistent with signal and membrane anchor regions respectively, and 11 potential sites for N-glycosylation. Alignment of the amino acid sequence with those published for HSV gH, varicella zoster virus gpIII, Epstein Barr virus gp85 and human cytomegalovirus p86 shows similarity of the EHV gene with the 2 other alpha-herpesviruses over most of the polypeptide, but only the C-terminal half could be aligned for all 5 viruses. The identical positioning of 6 cysteine residues and a number of highly conserved amino acid motifs supports a common evolutionary origin of this gene and is consistent with its role as an essential glycoprotein of the herpesvirus family. An origin of replication is predicted to occur at approximately 300 nucleotides downstream of the EHV-1 gH coding region, on the basis of similarity to other herpesvirus origins.
通过核苷酸及其推导的氨基酸序列，鉴定并表征了马疱疹病毒1型（EHV - 1，马流产病毒）中一个与单纯疱疹病毒（HSV）糖蛋白H基因同源的基因。EHV - 1 gH基因位于0.47 - 0.49图谱单位处，包含一个开放阅读框，能够编码一个由848个氨基酸组成的多肽，分别包括与信号和膜锚定区域一致的N端和C端疏水结构域，以及11个潜在的N - 糖基化位点。将该氨基酸序列与已发表的HSV gH、水痘带状疱疹病毒gpIII、爱泼斯坦 - 巴尔病毒gp85和人巨细胞病毒p86的氨基酸序列进行比对，结果显示EHV基因在多肽的大部分区域与另外两种α疱疹病毒相似，但所有5种病毒仅C端的一半能够比对。6个半胱氨酸残基的相同定位和一些高度保守的氨基酸基序支持了该基因的共同进化起源，并与其作为疱疹病毒家族必需糖蛋白的作用相一致。基于与其他疱疹病毒起源的相似性，预计在EHV - 1 gH编码区域下游约300个核苷酸处会出现一个复制起点。
