Huang L C, Lius S, Huang B L, Murashige T, Mahdi el F M, Van Gundy R
Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan.
Plant Physiol. 1992 Jan;98(1):166-73. doi: 10.1104/pp.98.1.166.
Repeated grafting of 1.5-centimeter long shoot tips from an adult Sequoia sempervirens tree onto fresh, rooted juvenile stem cuttings in vitro resulted in progressive restoration of juvenile traits. After four successive grafts, stem cuttings of previously adult shoots rooted as well, branched as profusely, and grew with as much or more vigor as those of seedling shoots. Reassays disclosed retention for 3 years of rooting competence at similar levels as originally restored. Adventitious shoot formation was remanifested and callus development was depressed in stem segments from the repeatedly grafted adult. The reversion was associated with appearance and disappearance of distinctive leaf proteins. Neither gibberellic acid nor N(6)-beneyladenine as nutrient supplements duplicated the graft effects.
将成年北美红杉树1.5厘米长的茎尖反复嫁接到新鲜的、已生根的幼年茎段插条上进行离体培养，结果幼年性状逐渐恢复。经过连续四次嫁接后，先前成年枝条的茎段插条也能生根，分枝同样繁茂，生长活力与幼苗枝条相当甚至更强。重新检测发现，生根能力在3年内保持在与最初恢复时相似的水平。反复嫁接的成年植株的茎段中，不定芽形成再次出现，愈伤组织发育受到抑制。这种逆转与独特叶片蛋白质的出现和消失有关。作为营养补充剂的赤霉素和N(6)-苄基腺嘌呤都无法复制嫁接效果。
