McGarvey D J, Sirevåg R, Christoffersen R E
Department of Biological Sciences, University of California, Santa Barbara, California 93106.
Plant Physiol. 1992 Feb;98(2):554-9. doi: 10.1104/pp.98.2.554.
Fruit ripening involves a series of changes in gene expression regulated by the phytohormone ethylene. AVOe3, a ripening-related gene in avocado fruit (Persea americana Mill. cv Hass), was characterized with regard to its ethylene-regulated expression. The AVOe3 mRNA and immunopositive protein were induced in mature fruit within 12 hours of propylene treatment. The AVOe3 mRNA levels reached a maximum 1 to 2 days before the ethylene climacteric, whereas the immunopositive protein continued to accumulate. RNA selected by the pAVOe3 cDNA clone encoded a polypeptide with molecular mass of 34 kilodaltons, corresponding to the molecular mass of the AVOe3 protein determined by immunoblots. The protein was soluble, remaining in solution at 100,000 gravity and eluted as a monomer on gel filtration. Because of its pattern of induction and relationship to an ethylene-related gene of tomato, the possible involvement of AVOe3 in ethylene biosynthesis is discussed.
果实成熟涉及一系列由植物激素乙烯调控的基因表达变化。AVOe3是鳄梨果实（Persea americana Mill. cv Hass）中一个与成熟相关的基因，对其乙烯调控表达进行了表征。在丙烯处理12小时内，成熟果实中诱导出了AVOe3 mRNA和免疫阳性蛋白。AVOe3 mRNA水平在乙烯跃变前1至2天达到最大值，而免疫阳性蛋白则持续积累。由pAVOe3 cDNA克隆筛选出的RNA编码一种分子量为34千道尔顿的多肽，这与免疫印迹法测定的AVOe3蛋白分子量相对应。该蛋白可溶，在100,000重力下仍保留在溶液中，并在凝胶过滤时以单体形式洗脱。由于其诱导模式以及与番茄乙烯相关基因的关系，讨论了AVOe3在乙烯生物合成中的可能作用。
