MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1989 Sep;91(1):1-4. doi: 10.1104/pp.91.1.1.
The properties of enzymes involved in ethylene biosynthesis are reviewed and progress toward the purification of these enzymes is described. The enzyme whose activity usually limits ethylene biosynthesis is 1-aminocyclopropane-1-carboxylate (ACC) synthase. Even though its level in plants is extremely low, it has now been purified from several sources. The enzyme that converts ACC to ethylene does not survive homogenization, apparently because it is membrane-bound and because its activity requires membrane integrity. Properties of this enzyme have been elucidated in vivo and in vacuolar preparations which possess the capacity to convert ACC to ethylene.
本文综述了参与乙烯生物合成的酶的性质,并描述了这些酶的纯化进展。通常限制乙烯生物合成的酶是 1-氨基环丙烷-1-羧酸(ACC)合酶。尽管其在植物中的含量极低,但现已从多种来源中纯化出来。将 ACC 转化为乙烯的酶在匀浆过程中不能存活,显然是因为它是膜结合的,而且其活性需要膜的完整性。该酶的性质已经在体内和具有将 ACC 转化为乙烯能力的液泡制剂中阐明。