Julius-von-Sachs-Institut für Biowissenschaften der Universität Würzburg, Mittlerer Dallenbergweg 64, D-8700 Würzburg, Federal Republic of Germany.
Plant Physiol. 1992 Feb;98(2):738-44. doi: 10.1104/pp.98.2.738.
After exposing intact chloroplasts isolated from spinach (Spinacia oleracea L. cv Yates) and capable of photoreducing CO(2) at high rates to different concentrations of radioactive sulfite in the light or in the dark, (35)SO(2) and H(2) (35)S were removed from the acidified suspensions in a stream of nitrogen. Remaining activity could be fractionated into sulfate, organic sulfides, and sulfite addition compounds. When chloroplast suspensions contained catalase, superoxide dismutase and O-acetylserine, the oxidation of sulfite to sulfate was slower in the light than the reductive formation of sulfides that exhibited a maximum rate of about 2 micromoles per milligram chlorophyll per hour, equivalent to about 1% of maximum carbon assimilation. Botht the oxidative and the reductive detoxification of sulfite were very slow in the dark. Oxidation was somewhat, but not much, accelerated in the light in the absence of O-acetylserine, which caused a dramatic decrease in the formation of organic sulfides and an equally dramatic increase in the concentration of sulfite addition compounds whose formation was light-dependent. The sulfite addition compounds were not identified. Addition compounds did not accumulate in the dark. In the light, the electron transport inhibitor 3-(3,4-dichlorophenyl)-1,1-dimethylurea, diuron, decreased not only the reduction, but also the oxidation of sulfite and the formation of addition compounds.
将菠菜（Spinacia oleracea L. cv Yates）完整的叶绿体分离出来，并在光照或黑暗条件下，将其暴露于不同浓度的放射性亚硫酸盐中，这些叶绿体能够高效地还原 CO(2)。然后，用氮气将酸化的悬浮液中的 (35)SO(2)和 H(2) (35)S 去除。剩余的活性物质可以被分离成硫酸盐、有机硫化物和亚硫酸盐加成化合物。当叶绿体悬浮液中含有过氧化氢酶、超氧化物歧化酶和 O-乙酰丝氨酸时，亚硫酸盐的氧化速度在光照下比硫化物的还原形成速度慢，最大速率约为每毫克叶绿素每小时 2 微摩尔，相当于最大碳同化的 1%左右。在黑暗中，亚硫酸盐的氧化和还原解毒都非常缓慢。在没有 O-乙酰丝氨酸的情况下，光照会稍微加速氧化，但不会太多，这会导致有机硫化物的形成急剧减少，同时亚硫酸盐加成化合物的浓度急剧增加，其形成依赖于光照。亚硫酸盐加成化合物没有被鉴定出来。加成化合物在黑暗中不会积累。在光照下，电子传递抑制剂 3-(3,4-二氯苯基)-1,1-二甲基脲、敌草隆不仅会降低亚硫酸盐的还原，还会降低其氧化和加成化合物的形成。
