Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1992 May;99(1):197-202. doi: 10.1104/pp.99.1.197.
Two different mutants of Arabidopsis thaliana deficient in chloroplast membrane lipid polyunsaturation were indistinguishable in appearance from the wild-type when grown at 22 degrees C. By contrast, leaf tissues of the mutants that developed during growth at 5 degrees C were chlorotic, whereas the wild type was not. This is the first direct evidence that chloroplast lipid polyunsaturation contributes to low-temperature fitness. Chloroplasts from mutant lines grown at 5 degrees C were much smaller than those of the wild-type, and the thylakoid membrane content was reduced by up to 70%. However, there was no discernible effect of low temperature on chloroplasts that developed prior to exposure to low temperatures. These and related observations suggest that the high degree of chloroplast membrane lipid polyunsaturation is required for some aspect of chloroplast biogenesis.
当在 22°C 下生长时，两种拟南芥叶绿体膜脂质不饱和的不同突变体在外观上与野生型没有区别。相比之下，在 5°C 下生长的突变体的叶片组织呈黄化，而野生型则没有。这是直接证明叶绿体脂质不饱和有助于适应低温的第一个证据。在 5°C 下生长的突变体的叶绿体比野生型小得多，类囊体膜含量减少了多达 70%。然而，低温对暴露于低温之前发育的叶绿体没有明显的影响。这些和相关的观察结果表明，叶绿体膜脂质的高度不饱和是叶绿体生物发生的某个方面所必需的。
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