Vertucci C W
U.S. Department of Agriculture-Agricultural Research Station, Fort Collins, Colorado 80523.
Plant Physiol. 1992 May;99(1):310-6. doi: 10.1104/pp.99.1.310.
Changes in the lipid constituents of seeds are believed to be associated with losses of seed viability during storage. The physical properties of lipids in seeds of genetically similar backgrounds but varying ages were compared using differential scanning calorimetry. The technique measures the temperature and energy associated with lipid-melting transitions and is noninvasive. Although differences in the temperature at which lipids melted were sometimes observed among deteriorated and fresh seeds, the direction of the change was inconsistent among species tested. For all species tested except tomato, there was a decrease in the energy associated with the lipid melt in deteriorated samples, and the change occurred at a similar rate as the loss of seed vigor. The data suggest that there are changes in the lipid components of seeds that are associated with seed deterioration and that these changes can be measured using differential scanning calorimetry.
种子脂质成分的变化被认为与储存期间种子活力的丧失有关。使用差示扫描量热法比较了遗传背景相似但年龄不同的种子中脂质的物理性质。该技术测量与脂质熔化转变相关的温度和能量,并且是非侵入性的。虽然有时在劣变种子和新鲜种子之间观察到脂质熔化温度的差异,但在所测试的物种中变化方向并不一致。对于除番茄以外的所有测试物种,劣变样品中与脂质熔化相关的能量都有所下降,并且这种变化与种子活力丧失的速率相似。数据表明,种子的脂质成分存在与种子劣变相关的变化,并且这些变化可以使用差示扫描量热法进行测量。