Institute of Biology, National Center for Scientific Research of Vietnam, Nghia Do, Tu Liem, Hanoi, Vietnam.
Plant Physiol. 1992 Jul;99(3):1146-50. doi: 10.1104/pp.99.3.1146.
Polyadenylated RNA was isolated and a cDNA library constructed from seedlings of a chilling-tolerant rice cultivar (Oryza sativa L. subsp. Japonica cv Nipponbare). Four clones were isolated by differential screening. Northern blot hybridization using RNAs from chilling-tolerant (Nipponbare) and -sensitive (IR36) cultivars revealed higher steady-state levels of transcripts for the four genes in Nipponbare than in IR36 maintained at the same low temperatures. The accumulation of transcripts homologous to selected cDNA sequences during chilling were tissue-specific. The nucleotide and deduced amino acid sequences of three clones, pBC121, pBC442, and pBC591, were determined, and no homology was identified by comparison with the latest version of EMBL and LASL gene data bases. The deduced protein sequences from the longest open reading frame of the clones pBC121 and pBC442 are rich in leucine and serine, whereas that of the clone pBC591 contains arginine-rich basic domains.
聚腺苷酸化 RNA 被分离出来，并且从耐冷水稻品种（Oryza sativa L. subsp. Japonica cv Nipponbare）的幼苗中构建了 cDNA 文库。通过差异筛选分离出了四个克隆。用来自耐冷（Nipponbare）和敏感（IR36）品种的 RNA 进行 Northern blot 杂交表明，在相同的低温下，Nipponbare 中四个基因的转录本的稳定水平高于 IR36。在冷胁迫过程中，与选定 cDNA 序列同源的转录本的积累具有组织特异性。三个克隆 pBC121、pBC442 和 pBC591 的核苷酸和推导的氨基酸序列被确定，并且与 EMBL 和 LASL 基因数据库的最新版本进行比较未发现同源性。克隆 pBC121 和 pBC442 的最长开放阅读框的推导蛋白序列富含亮氨酸和丝氨酸，而克隆 pBC591 的则含有富含精氨酸的碱性结构域。
