Hosaka Y, Akahori H, Hernandez F, Akaike K, Ikegami N
Department of Microbiology, Osaka University of Pharmaceutical Sciences, Japan.
J Electron Microsc (Tokyo). 1991 Dec;40(6):407-10.
Fragments of disrupted capsids were frequently seen in purified preparations of human rotavirus inner capsids by negative staining method in electron microscopy. The ultrastructures of these fragments were analyzed in comparison with the T = 13L model. Well-resolved pentamers were rarely seen in the fragments on observation at the standard electron doses, but were frequently seen at low electron doses, which meant a better preservation of the steric structure of capsid fragments in the latter case. Thus advantages of low dose electron microscopy have been shown in observation of capsid fragments.
在电子显微镜下，通过负染色法在人轮状病毒内衣壳的纯化制剂中经常可见破裂衣壳的片段。与T = 13L模型相比，对这些片段的超微结构进行了分析。在标准电子剂量下观察时，片段中很少见到分辨率良好的五聚体，但在低电子剂量下经常见到，这意味着在后一种情况下衣壳片段的空间结构保存得更好。因此，低剂量电子显微镜在观察衣壳片段方面已显示出优势。
