Fricker Mark, Runions John, Moore Ian
Department of Plant Sciences, University of Oxford, Oxford, OX1 3RB England.
Annu Rev Plant Biol. 2006;57:79-107. doi: 10.1146/annurev.arplant.57.032905.105239.
A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present in three-, four-, or even five-dimensional images into statistically useful measurements. This review explores a range of in vivo fluorescence imaging applications in plants, with particular emphasis on where quantitative techniques are beginning to emerge.
已经开发出大量精妙的实验方法来对活植物细胞中的DNA、mRNA、蛋白质、细胞器、代谢物和离子的分布及动态进行成像。尽管人脑能够快速吸收视觉信息，尤其是当以动画和影片形式呈现时，但要将三维、四维甚至五维图像中呈现的海量数据浓缩成具有统计学意义的有用测量值则更具挑战性。本综述探讨了一系列植物体内荧光成像应用，特别强调了定量技术开始出现的领域。
