Krasheninnikova L V, Rassadina G V, Kirsanova S N, Khromova L M, Iusibov V M, Pak Chun I r, Andrianov V M, Piruzian E S
Mol Gen Mikrobiol Virusol. 1991 Nov(11):17-20.
The genetic constructions based on integrative vector pGV3850 were used to introduce bacterial genes xyl and T-cyt into potato cells. The transformation was carried out using the leaf-disc method with modifications. A special system for obtaining regenerants from explants of potato in vitro plants or calli has been designed that permitted the selection of transgenic shoots. The presence of the genes in potato genome has been proved by testing the NPTII and glucoisomerase activities. The transgenic plants expressing T-cyt gene differed from the wild type in sharp decrease of the apical dominance.
基于整合载体pGV3850构建的基因结构被用于将细菌基因xyl和T-cyt导入马铃薯细胞。采用改良的叶盘法进行转化。设计了一种从马铃薯离体植株外植体或愈伤组织中获得再生植株的特殊系统，该系统可用于筛选转基因芽。通过检测NPTII和葡萄糖异构酶活性，证实了这些基因存在于马铃薯基因组中。表达T-cyt基因的转基因植株与野生型植株的区别在于顶端优势明显降低。
