Padegimas L, Shul'ga O A, Skriabin K G
Mol Biol (Mosk). 1994 Mar-Apr;28(2):437-43.
The expression cassette for phosphinothricin acetyltransferase gene (PAT or bar) from S. hygroscopicus has been constructed on the basis of the pBI121 plasmid. Leaf disks of N. tabacum cv. SR1 and steam segments of S. tuberosum cv. Prigozhii-2 have been transformed using Agrobacterium. Selection of the transformed plants carried out by PCR analysis revealed 12 transgenic tobacco plants and 3 transgenic potato plants. bar gene expression and plant resistance to phosphinothricin treatment have been studied in regenerated plants. All the transgenic plants were found to be resistant to the herbicide. Mendelian inheritance of the inserted gene has been proved by analysis of the F1 progeny of transformed tobacco plants.
基于pBI121质粒构建了吸水链霉菌的膦丝菌素乙酰转移酶基因（PAT或bar）表达盒。利用农杆菌对烟草品种SR1的叶盘和马铃薯品种Prigozhii - 2的茎段进行了转化。通过PCR分析对转化植株进行筛选，结果显示有12株转基因烟草植株和3株转基因马铃薯植株。对再生植株进行了bar基因表达及植株对膦丝菌素处理抗性的研究。发现所有转基因植株均对除草剂具有抗性。通过对转基因烟草植株F1代的分析，证明了插入基因的孟德尔遗传。
