Quan S F, Witten M L, Dambro N N, Lemen R J
Department of Internal Medicine, University of Arizona College of Medicine, Tucson 85724.
Prostaglandins Leukot Essent Fatty Acids. 1991 Nov;44(3):171-5. doi: 10.1016/0952-3278(91)90052-7.
Acute infections in beagle puppies with canine parainfluenza virus type 2 (CP12), and CP12 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A2 and prostacylin, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) respectively, and leukotriene B4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 +/- 1 days, mean +/- SEM) 3-4 days after no infection (control, n = 6), inoculation with both CP12 and Bb (CP12-Bb, n = 11), inoculation with CP12 alone (CP12, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB2 and 6-keto-PGF1 alpha were measured before and after infection in the CP12-Bb and control groups. The BAL concentration of thromboxane B2 was increased in the CP12-Bb group (520 +/- 120 pg/ml), but not in the CP12 (88 +/- 40 pg/ml), Bb (235 +/- 100 pg/ml), or control groups (120 +/- 60 pg/ml, p less than 0.01). There also was a borderline increase in BAL concentration of LTB4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF1 alpha. Furthermore, neither TXB2 nor PGF1 alpha was elevated in the plasma of control or CP12-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CP12-Bb group.
2型犬副流感病毒（CP12）感染比格幼犬，以及CP12与支气管败血波氏杆菌（Bb）联合感染，在急性感染期间会导致细支气管炎，并增加气道对雾化组胺的反应性。为了确定这些观察结果是否与类花生酸水平升高有关，分别测量了25只比格幼犬（年龄 = 76 ± 1天，平均值 ± 标准误）在未感染（对照组，n = 6）、接种CP12和Bb（CP12 - Bb，n = 11）、单独接种CP12（CP12，n = 4）和单独接种Bb（Bb，n = 4）3 - 4天后支气管肺泡灌洗（BAL）液中血栓素A2和前列环素的稳定代谢产物血栓素B2（TXB2）和6 - 酮 - 前列腺素F1α（6 - 酮 - PGF 1α）以及白三烯B4的水平。此外，还测量了CP12 - Bb组和对照组感染前后血浆中TXB2和6 - 酮 - PGF1α的水平。CP12 - Bb组BAL液中血栓素B2的浓度升高（520 ± 120 pg/ml），但CP12组（88 ± 40 pg/ml）、Bb组（235 ± 100 pg/ml）或对照组（120 ± 60 pg/ml，p < 0.01）未升高。CP12 - Bb组BAL液中白三烯B4的浓度也有临界升高。6 - 酮 - PGF1α的BAL浓度未观察到差异。此外，对照组或CP12 - Bb组幼犬的血浆中TXB2和PGF1α均未升高。这些数据表明，CP12 - Bb组急性感染期间BAL液中血栓素浓度升高与组胺高反应性有关。
