Teglas Mike B, Drazenovich Nicole L, Stott Jeff, Foley Janet E
Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Vet Parasitol. 2006 Sep 10;140(3-4):327-33. doi: 10.1016/j.vetpar.2006.03.027. Epub 2006 May 2.
Epizootic bovine abortion (EBA), also known as "foothill abortion", is a vector borne disease of beef cattle that graze in the mountainous regions of California, southern Oregon and western Nevada transmitted by the argasid tick Ornithodoros coriaceus. Recently, the putative agent of EBA was identified as a novel Deltaproteobacter in the order Myxococcales. In this study, a TaqMan real-time PCR (TM-PCR) protocol specific to the putative EBA agent was developed. The new real-time TM-PCR assay functioned sensitively and specifically to detect pathogen DNA in field-collected O. coriaceus ticks. The assay had an analytical sensitivity of a single plasmid copy and, when evaluated with a collection of tick-borne pathogens, yielded a positive PCR-result only for the agent of EBA. Use of the TM-PCR represents an effective tool for rapid and highly sensitive assessment of environmental risk and spatial and statistical analysis to highlight areas where there may be increased risk for EBA in susceptible cattle.
流行性牛流产（EBA），也被称为“山麓流产”，是一种由蜱传播的肉牛疾病，发生于在加利福尼亚州山区、俄勒冈州南部和内华达州西部放牧的牛群中，由软蜱科的革蜱传播。最近，EBA的假定病原体被鉴定为黏球菌目中的一种新型δ变形菌。在本研究中，开发了一种针对假定的EBA病原体的TaqMan实时PCR（TM-PCR）方法。新的实时TM-PCR检测方法灵敏且特异，可检测野外采集的革蜱中的病原体DNA。该检测方法的分析灵敏度为单拷贝质粒，在用一系列蜱传播的病原体进行评估时，仅对EBA病原体产生阳性PCR结果。TM-PCR的应用是一种有效的工具，可用于快速、高度灵敏地评估环境风险以及进行空间和统计分析，以突出易感牛群中EBA风险可能增加的区域。
