Naranjo Victoria, Ayoubi Patricia, Vicente Joaquín, Ruiz-Fons Francisco, Gortazar Christian, Kocan Katherine M, de la Fuente José
Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13003 Ciudad Real, Spain.
Vet Microbiol. 2006 Aug 25;116(1-3):224-31. doi: 10.1016/j.vetmic.2006.03.013. Epub 2006 May 2.
Bovine tuberculosis (bTB), caused by Mycobacterium bovis (Mycobacterium tuberculosis complex), is a zoonotic disease that affects cattle and wildlife worldwide. These animal hosts can serve as reservoirs of infection, thus increasing the risk of human exposure and infection. In this study we quantified by RNA macroarray fluorescent hybridization and real-time RT-PCR the mRNA levels of genes differentially expressed in oropharyngeal tonsils and mandibular lymph nodes of three and seven individual non-tuberculous and tuberculous wild boars naturally exposed to M. bovis, respectively. These results demonstrated upregulation of two genes, complement component 3 (C3) and methylmalonyl-CoA mutase (MUT), in the non-tuberculous wild boars. These upregulated genes may contribute to resistance of wild boars to bTB by modifying the innate immunity, which limits the ability of the mycobacterium to infect and persist within macrophages. The C3 and MUT genes, therefore, are likely to be good candidates to study as markers of bTB resistance using functional genomics in animal model systems. Identification of genes upregulated in wild animals resistant to bTB contributes to our understanding of the mechanisms of protective immunity and resistance to mycobacterial organisms.
牛结核病（bTB）由牛分枝杆菌（结核分枝杆菌复合群）引起，是一种人畜共患病，影响着全球的牛和野生动物。这些动物宿主可作为感染源，从而增加人类接触和感染的风险。在本研究中，我们通过RNA宏阵列荧光杂交和实时逆转录聚合酶链反应，分别对三头和七头自然感染牛分枝杆菌的非结核和结核野生公猪的口咽扁桃体和下颌淋巴结中差异表达基因的mRNA水平进行了定量分析。这些结果表明，在非结核野生公猪中，补体成分3（C3）和甲基丙二酰辅酶A变位酶（MUT）这两个基因上调。这些上调的基因可能通过改变固有免疫来增强野猪对牛结核病的抵抗力，固有免疫可限制分枝杆菌在巨噬细胞内感染和存活的能力。因此，C3和MUT基因很可能是利用动物模型系统中的功能基因组学研究牛结核病抗性标志物的良好候选基因。鉴定出在对牛结核病有抗性的野生动物中上调的基因，有助于我们理解保护性免疫和对分枝杆菌生物体抗性的机制。
