Lastra José Manuel Pérez de la, Galindo Ruth C, Gortázar Christian, Ruiz-Fons Francisco, Aranaz Alicia, de la Fuente José
Instituto de Investigación en Recursos Cinegéticos, Ronda Toledo s/n, 13005 Ciudad Real, Spain.
Vet Microbiol. 2009 Mar 2;134(3-4):334-9. doi: 10.1016/j.vetmic.2008.08.026. Epub 2008 Aug 29.
The objective of this study was to analyze the expression of immunoregulatory genes in European wild boar (Sus scrofa) immunized with BCG. Eighteen immunoregulatory genes were selected for expression analysis based on their role in host immune response during tuberculosis and/or for their association with resistance to bovine tuberculosis in European wild boar populations. Initially, mRNA levels were analyzed by quantitative real-time reverse transcription PCR (qRT-PCR) in spleen samples from Mycobacterium bovis-infected (N=18) and uninfected (N=22) European wild boar. Statistical analysis of qRT-PCR data revealed that four genes, complement component C3, IFN-gamma, IL-4 and RANTES were downregulated in infected animals (P<0.05). These genes were selected for analysis of mRNA levels in peripheral blood mononuclear cells (PBMCs) from seven wild boar experimentally immunized with BCG and seven non-immunized controls. Blood was collected at 0, 5, 13 and 25 weeks post-immunization (wpi). The mRNA levels of IFN-gamma and C3 showed a peak (>15-fold increase) at 5 wpi, whereas transcripts for RANTES and IL-4 showed a peak (>2-fold increase) at 13 wpi in BCG-immunized animals when compared to non-immunized controls. The pattern of expression of these genes over the time provides the first description of BCG specific immune response in European wild boar. These results provide new insights into the molecular basis of wild boar response to M. bovis infection and BCG vaccination and may be used to monitor BCG vaccination in this species.
本研究的目的是分析用卡介苗免疫的欧洲野猪（Sus scrofa）中免疫调节基因的表达。基于18种免疫调节基因在结核病宿主免疫反应中的作用和/或它们与欧洲野猪群体对牛结核病抗性的关联，选择这些基因进行表达分析。最初，通过定量实时逆转录PCR（qRT-PCR）分析来自感染牛分枝杆菌（N = 18）和未感染（N = 22）的欧洲野猪脾脏样本中的mRNA水平。qRT-PCR数据的统计分析显示，感染动物中补体成分C3、干扰素-γ、白细胞介素-4和调节激活正常T细胞表达和分泌因子（RANTES）这四个基因表达下调（P<0.05）。选择这些基因分析来自七只经卡介苗实验免疫的野猪和七只未免疫对照的外周血单个核细胞（PBMC）中的mRNA水平。在免疫后0、5、13和25周（wpi）采集血液。与未免疫对照相比，卡介苗免疫动物中干扰素-γ和C3的mRNA水平在5 wpi时出现峰值（增加超过15倍），而RANTES和白细胞介素-4的转录本在13 wpi时出现峰值（增加超过2倍）。这些基因随时间的表达模式首次描述了欧洲野猪对卡介苗的特异性免疫反应。这些结果为野猪对牛分枝杆菌感染和卡介苗接种反应的分子基础提供了新的见解，并可用于监测该物种的卡介苗接种情况。
