Valli V E, Vernau W, de Lorimier L-P, Graham P S, Moore P F
Department of Pathobiology, College of Veterinary Medicine, University of Illinois, Room 284 SAC, 1008 W Hazelwood Dr., Urbana, IL, 61802, USA.
Vet Pathol. 2006 May;43(3):241-56. doi: 10.1354/vp.43-3-241.
Sixty-six cases of indolent canine lymphoid proliferation were reviewed. Age ranged from 1.5 to 16 years (median 9.0 years). Dogs of 26 breeds, plus 13 of mixed breeding or unknown lineage, were represented. B-Cell lymphomas (CD79a+) predominated. Marginal zone lymphoma (MZL), the largest group, involved lymph node (33 cases) and spleen (13 cases), with both tissues involved in five of these cases. Follicular lymphoma (FL) involved lymph nodes (five cases), and mantle cell lymphoma (MCL) occurred as solitary splenic masses (three cases). Nodal CD3+ T-zone lymphomas (TZL) (10 cases), were included since they resembled late-stage MZL at the architectural level. Two cases of marginal zone hyperplasia (MZH) were included to aid in differentiation of early MZL. Clonality status was determined in 54 cases by analysis of immunoglobulin heavy chain (IGH) and T-cell antigen receptor gamma (TCRG) gene rearrangement. Clonal rearrangement of IGH was detected in 28 of 35 MZL cases (80%), four of four FL cases (100%) and three of three MCL cases (100%). Concurrent cross lineage rearrangement of TCRG was detected in six MZL and two FL cases. Clonal rearrangement of TCRG was documented in five of eight TZL cases (63%). Limited survival data obtained for 18 dogs indicated that the B-cell lymphomas (MZL, MCL, and FL) and the T-cell lymphoma (TZL) were associated with indolent behavior and long survival. Although to the authors' knowledge, the true incidence of canine indolent lymphomas is unknown, the tumors are not rare and may have been underrecognized. Recognition of their architectural features, routine application of immunophenotyping, and molecular clonality assessment should alleviate this.
回顾了66例惰性犬类淋巴增殖病例。年龄范围为1.5至16岁（中位数9.0岁）。涵盖了26个品种的犬，另外还有13只混种或谱系不明的犬。B细胞淋巴瘤（CD79a+）占主导。边缘区淋巴瘤（MZL）是最大的组，累及淋巴结（33例）和脾脏（13例），其中5例同时累及这两个组织。滤泡性淋巴瘤（FL）累及淋巴结（5例），套细胞淋巴瘤（MCL）表现为孤立的脾脏肿块（3例）。结节性CD3+ T区淋巴瘤（TZL）（10例）也包括在内，因为它们在结构水平上类似于晚期MZL。纳入了2例边缘区增生（MZH）以辅助早期MZL的鉴别。通过分析免疫球蛋白重链（IGH）和T细胞抗原受体γ（TCRG）基因重排，确定了54例病例的克隆状态。在35例MZL病例中的28例（80%）、4例FL病例中的4例（100%）和3例MCL病例中的3例（100%）检测到IGH的克隆重排。在6例MZL和2例FL病例中检测到TCRG的同时跨谱系重排。在8例TZL病例中的5例（63%）记录到TCRG的克隆重排。对18只犬获得的有限生存数据表明，B细胞淋巴瘤（MZL、MCL和FL）和T细胞淋巴瘤（TZL）与惰性行为和长生存期相关。尽管据作者所知，犬惰性淋巴瘤的真实发病率尚不清楚，但这些肿瘤并不罕见，可能一直未得到充分认识。认识它们的结构特征、常规应用免疫表型分析和分子克隆性评估应能缓解这一情况。
