Dittrich R, Maltaris T, Mueller A, Dimmler A, Hoffmann I, Kiesewetter F, Beckmann M W
Department of Obstetrics and Gynecology, Erlangen University Hospital, Germany.
Horm Metab Res. 2006 Mar;38(3):141-5. doi: 10.1055/s-2006-925175.
Cryopreservation of whole organs would not only be advantageous for experimental biology but also for transplantation surgery. The objective of this study was to evaluate the feasibility of cryopreserving swine uterus as an experimental model of cryopreservation affecting a whole visceral muscle organ. Organs were frozen slowly (0.2 degrees C/min) after arterial perfusion with 10% dimethyl sulfoxide solution for 10 min and equilibration in this solution for 30 min. Viability of the organs was tested by biochemical parameters, LIVE/DEAD fluorescent staining and capability for contraction in a perfusion system. Ten fresh and ten cryopreserved uteri showed living cells in the LIVE/DEAD assay, and were viable for at least 7 h as shown by their ability to produce rhythmic contractions after oxytocin administration. This is the first report known to us on successful cryopreservation of a whole swine uterus to demonstrate that perfusion with a cryoprotectant and slow freezing provides a promising method for cryopreserving whole organs, and may encourage further studies with other cryoprotective agents and freezing protocols as well as other organs.
全器官的冷冻保存不仅对实验生物学有利，对移植手术也有益。本研究的目的是评估将猪子宫作为影响整个内脏肌性器官的冷冻保存实验模型进行冷冻保存的可行性。在用10%二甲亚砜溶液进行动脉灌注10分钟并在该溶液中平衡30分钟后，器官被缓慢冷冻（0.2℃/分钟）。通过生化参数、活/死荧光染色以及在灌注系统中的收缩能力来测试器官的活力。十个新鲜子宫和十个冷冻保存的子宫在活/死检测中显示有活细胞，并且在给予催产素后能够产生节律性收缩，这表明它们至少存活了7小时。这是我们所知的关于成功冷冻保存整个猪子宫的第一份报告，证明用冷冻保护剂灌注和缓慢冷冻为冷冻保存全器官提供了一种有前景的方法，并且可能会鼓励对其他冷冻保护剂、冷冻方案以及其他器官进行进一步研究。
