Dittrich R, Beckmann M W, Mueller A, Binder H, Hoffmann I, Maltaris T
Department of Obstetrics and Gynecology, Erlangen University Hospital, University of Erlangen-Nuremberg, Erlangen, Germany.
Reprod Domest Anim. 2010 Feb;45(1):86-91. doi: 10.1111/j.1439-0531.2008.01258.x. Epub 2008 Oct 16.
Cryopreservation of cells and even tissue is feasible. New exciting findings arise in the promising field of cryobiology, e.g. the cryopreservation of whole ovaries. Uterus cryopreservation would be advantageous not only for experimental biology, but also for transplantation surgery. The objective of this study was to evaluate various cryopreservation protocols as well as various storage temperatures in cryopreservation of whole swine uteri. The used freezing protocol was slow (0.2 degrees C/min) after arterial perfusion with 1%, 5% or 10% dimethyl sulfoxide (DMSO) solution for 10 min and equilibration in this solution for 30 min. Viability of the organs was tested by histological examination, biochemical parameters and by the capability of rhythmical contractions in a perfusion system. Eighty swine uteri were cryopreserved. All uteri that were frozen with 10% and 5% DMSO were viable after thawing for at least 1 h, whereas only 40% survived with the use of 1% DMSO and 0% with the use of 0.5% DMSO, respectively. There was no difference regarding the survival rates after various cryostorage periods for up to 16 weeks or after cryostorage for 2 weeks in -70 degrees C or -130 degrees C. The cryopreservation of a whole organ such as the swine uterus is a valuable method for the study of cryoprotective agents and freezing protocols. This study demonstrates clearly that the perfusion of the organ with cryoprotectants is the only factor which allows the uterus to contract.
细胞甚至组织的冷冻保存是可行的。在冷冻生物学这一充满前景的领域出现了一些令人兴奋的新发现，例如整个卵巢的冷冻保存。子宫冷冻保存不仅对实验生物学有利，对移植手术也有益。本研究的目的是评估各种冷冻保存方案以及在整个猪子宫冷冻保存中的不同储存温度。所采用的冷冻方案是在动脉灌注1%、5%或10%的二甲基亚砜（DMSO）溶液10分钟并在该溶液中平衡30分钟后进行缓慢冷冻（0.2℃/分钟）。通过组织学检查、生化参数以及在灌注系统中的节律性收缩能力来测试器官的活力。八十个猪子宫被冷冻保存。所有用10%和5%DMSO冷冻的子宫在解冻至少1小时后仍有活力，而分别使用1%DMSO时只有40%存活，使用0.5%DMSO时则无存活。在长达16周的不同冷冻保存期后或在-70℃或-130℃下冷冻保存2周后的存活率没有差异。整个器官如猪子宫的冷冻保存是研究冷冻保护剂和冷冻方案的一种有价值的方法。这项研究清楚地表明，用冷冻保护剂灌注器官是使子宫能够收缩的唯一因素。
