Qing W, Liu G
Zhonghua Yi Xue Za Zhi. 1991 Dec;71(12):694-6, 48.
The protective effect of DDB against carcinogen-induced DNA damage was examined in the present investigation. Preincubation of rat liver nuclei with DDB (1 mmol/L) resulted in inhibition of binding of 3H-benzo (a) pyrene to nuclear DNA. The inhibition rate was about 60%. Unscheduled DNA synthesis (UDS) of freshly isolated rat hepatocytes induced by aflatoxin B1 (10(-7) mol/L) was also dose dependently inhibited by DDB (10(-6)-10(-3) mol/L). Oral administration of DDB at 200 mg/kg once daily for 3 days was effective to induce increase of liver cytosol glutathione-S-transferase and microsomal UDPG-transferase in mice. The results indicate that DDB is able directly or indirectly to antagonize certain carcinogen-induced DNA damages.
本研究检测了联苯双酯（DDB）对致癌物诱导的DNA损伤的保护作用。用DDB（1 mmol/L）预孵育大鼠肝细胞核，可抑制3H-苯并(a)芘与核DNA的结合。抑制率约为60%。DDB（10(-6)-10(-3) mol/L）还剂量依赖性地抑制了黄曲霉毒素B1（10(-7) mol/L）诱导的新鲜分离大鼠肝细胞的非程序DNA合成（UDS）。小鼠每天口服200 mg/kg的DDB，连续3天，可有效诱导肝脏胞质谷胱甘肽-S-转移酶和微粒体UDPG-转移酶增加。结果表明，DDB能够直接或间接拮抗某些致癌物诱导的DNA损伤。
