嗜热水栖菌NTU103滚环复制质粒的特性分析
Characterization of a rolling-circle replication plasmid from Thermus aquaticus NTU103.
作者信息
Chu Sheng-Fen, Shu Hung-Yu, Lin Ling-Chun, Chen Mao-Yen, Tsay San-San, Lin Guang-Huey
机构信息
Microbial Genetics Laboratory, Institute of Microbiology, Immunology and Molecular Medicine, Tzu-Chi University, Hualien 970, Taiwan.
出版信息
Plasmid. 2006 Jul;56(1):46-52. doi: 10.1016/j.plasmid.2006.01.005. Epub 2006 May 3.
The thermophilic bacterium Thermus aquaticus NTU103 harbors a 1,965-bp plasmid, pTA103. Sequencing analysis revealed that pTA103 contains two open reading frames. One of the open reading frames (orf2) shares no significant homology with protein in the data bank. The other one has 50% similarity and 34% identity with RepA-like protein of pRm1132f, which is a rolling-circle replication (RCR) plasmid isolated from Sinorhizobium meliloti. S1 nuclease analysis demonstrated that pTA103 contains a single-stranded intermediate, confirming that pTA103 replicates via RCR mechanism. Sequence data also revealed putative double-stranded origin and single-stranded origin sites, indicating the importance of these cis elements in pTA103 replication.
嗜热栖热菌NTU103含有一个1965 bp的质粒pTA103。测序分析表明，pTA103含有两个开放阅读框。其中一个开放阅读框（orf2）与数据库中的蛋白质没有显著同源性。另一个与pRm1132f的RepA样蛋白有50%的相似性和34%的同一性，pRm1132f是从苜蓿中华根瘤菌中分离出的一种滚环复制（RCR）质粒。S1核酸酶分析表明，pTA103含有一个单链中间体，证实pTA103通过RCR机制复制。序列数据还揭示了假定的双链起点和单链起点位点，表明这些顺式元件在pTA103复制中的重要性。
Zotero 插件