苜蓿中华根瘤菌质粒pRm1132f通过滚环机制进行复制。
Sinorhizobium meliloti plasmid pRm1132f replicates by a rolling-circle mechanism.
作者信息
Barran L R, Ritchot N, Bromfield E S
机构信息
Soils and Crops Research and Development Centre, Agriculture and Agri-food Canada, Sainte-Foy, Quebec G1V 2J3, Canada.
出版信息
J Bacteriol. 2001 Apr;183(8):2704-8. doi: 10.1128/JB.183.8.2704-2708.2001.
Abstract
pRm1132f isolated from Sinorhizobium meliloti is a group III rolling-circle-replicating (RCR) plasmid. At least seven of eight open reading frames in the nucleotide sequence represented coding regions. The minimal replicon contained a rep gene and single- and double-stranded origins of replication. Detection of single-stranded plasmid DNA confirmed that pRm1132f replicated via an RCR mechanism.
摘要
从苜蓿中华根瘤菌中分离出的pRm1132f是一种III型滚环复制（RCR）质粒。核苷酸序列中的八个开放阅读框中至少有七个代表编码区。最小复制子包含一个rep基因以及单链和双链复制起点。单链质粒DNA的检测证实pRm1132f通过RCR机制进行复制。
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