植物乳杆菌 XY3 环型复制质粒 pXY3 的特性分析。
Characterization of a rolling-circle replication plasmid pXY3 from Lactobacillus plantarum XY3.
机构信息
Key Laboratory of Functional Dairy, Co-constructed by Ministry of Education and Beijing Municipality, College of Food Science & Nutritional Engineering, China Agricultural University, 17 Qinghua East Road, Haidian District, Beijing 100083, China.
出版信息
Plasmid. 2010 Jul;64(1):36-40. doi: 10.1016/j.plasmid.2010.03.003. Epub 2010 Mar 28.
The complete nucleotide sequence of cryptic plasmid pXY3 isolated from Lactobacillus plantarum strain XY3 has been determined. It consisted of a 2968-bp circular molecule with a G+C content of 39%. Sequence analysis of pXY3 revealed three putative open reading frames (ORFs). Based on sequence similarity, the Rep protein shared 89% and 88% identity with Rep proteins of pLF24 and pWCFS102, respectively, which belonged to the rolling-circle replication (RCR) pMV158 family. A ssoT-like single-strand origin (sso) and a typical pMV158 family double-strand origin (dso) located upstream of the rep gene. Southern blot analysis indicated pXY3 replicate via a rolling-circle (RC) mechanism. Furthermore, the relative copy number of pXY3 was estimated to be about 97 copies per chromosome equivalent by real-time PCR.
已确定从植物乳杆菌 XY3 菌株中分离出的隐秘质粒 pXY3 的完整核苷酸序列。它由一个 2968 碱基对的圆形分子组成，G+C 含量为 39%。pXY3 的序列分析显示了三个推定的开放阅读框 (ORF)。根据序列相似性，Rep 蛋白与分别属于滚环复制 (RCR) pMV158 家族的 pLF24 和 pWCFS102 的 Rep 蛋白具有 89%和 88%的同一性。ssoT 样单链起始 (sso) 和典型的 pMV158 家族双链起始 (dso) 位于 rep 基因的上游。Southern blot 分析表明，pXY3 通过滚环 (RC) 机制进行复制。此外，通过实时 PCR 估计 pXY3 的相对拷贝数约为每个染色体当量 97 个拷贝。
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