Yang Yu-Liang, Yang Feng-Ling, Jao Shu-Chuan, Chen Mao-Yen, Tsay San-San, Zou Wei, Wu Shih-Hsiung
Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.
J Lipid Res. 2006 Aug;47(8):1823-32. doi: 10.1194/jlr.M600034-JLR200. Epub 2006 May 4.
The structures of two major phosphoglycolipids from the thermophilic bacteria Thermus oshimai NTU-063, Thermus thermophilus NTU-077, Meiothermus ruber NTU-124, and Meiothermus taiwanensis NTU-220 were determined using spectroscopic and chemical analyses to be 2'-O-(1,2-diacyl-sn-glycero-3-phospho) -3'-O-(alpha-N-acetyl-glucosaminyl)-N-glyceroyl alkylamine [PGL1 (1)] and the novel structure 2'-O-(2-acylalkyldio-1-O-phospho)-3'-O-(alpha-N-acetylglucosaminyl)-N-glyceroyl alkylamine [PGL2 (2)]. PGL2 (2) is the first phosphoglycolipid identified with a 2-acylalkyldio-1-O-phosphate moiety. The fatty acids of the phosphoglycolipids are mainly iso-C(15:0), -C(16:0), and -C(17:0) and anteiso-C(15:0) and -C(17:0). The ratios of PGL2 (2) to PGL1 (1) are significantly altered when grown at different temperatures for three strains, T. thermophilus NTU-077, M. ruber NTU-124, and M. taiwanensis NTU-220, but not for T. oshimai NTU-063. Accordingly, the ratios of iso- to anteiso-branched fatty acids increase when grown at the higher temperature.
利用光谱分析和化学分析确定了嗜热细菌大岛栖热菌NTU - 063、嗜热栖热菌NTU - 077、红色栖热放线菌NTU - 124和台湾栖热放线菌NTU - 220中两种主要磷酸糖脂的结构，分别为2'-O-(1,2-二酰基-sn-甘油-3-磷酸)-3'-O-(α-N-乙酰氨基葡萄糖基)-N-甘油酰烷基胺[PGL1(1)]和新结构2'-O-(2-酰基烷基二氧-1-O-磷酸)-3'-O-(α-N-乙酰氨基葡萄糖基)-N-甘油酰烷基胺[PGL2(2)]。PGL2(2)是首个被鉴定含有2-酰基烷基二氧-1-O-磷酸部分的磷酸糖脂。这些磷酸糖脂的脂肪酸主要是异-C(15:0)、-C(16:0)和-C(17:0)以及anteiso-C(15:0)和-C(17:0)。对于嗜热栖热菌NTU - 077、红色栖热放线菌NTU - 124和台湾栖热放线菌NTU - 220这三株菌，当在不同温度下生长时，PGL2(2)与PGL1(1)的比例会显著改变，但大岛栖热菌NTU - 063不会。因此，在较高温度下生长时，异分支脂肪酸与anteiso分支脂肪酸的比例会增加。
