Lin Huei Ru, Lua Ahai Chang
Institute of Medical Sciences, Tzu Chi University, Hualien, Taiwan.
Rapid Commun Mass Spectrom. 2006;20(11):1724-30. doi: 10.1002/rcm.2499.
A method for the simultaneous determination of amphetamines and ketamines (ketamine, norketamine and dehydronorketamine) in urine samples by gas chromatography/mass spectrometry was developed and validated. Urine samples were extracted with organic solvent and derivatized with trifluoroacetic anhydride (TFAA). The limits of detection and limits of quantification for each analyte were lower than 19 and 30 ng/mL, respectively. Within-day and between-day precisions were within 0.5% and 10.6%, respectively. Biases for three levels of control samples were within -10.6% and +7.8%. The concentration of dehydronorketamine was greater than those of ketamine or norketamine in 19 of 35 ketamine-positive samples. A group of 110 human urine samples previously determined to contain at least one of the target analytes was analyzed using the new method, and excellent agreement was observed with previous results.
建立并验证了一种采用气相色谱/质谱法同时测定尿液样本中苯丙胺类和氯胺酮类（氯胺酮、去甲氯胺酮和脱氢去甲氯胺酮）的方法。尿液样本用有机溶剂萃取并用三氟乙酸酐（TFAA）衍生化。各分析物的检测限和定量限分别低于19 ng/mL和30 ng/mL。日内精密度和日间精密度分别在0.5%和10.6%以内。三个浓度水平对照样本的偏差在-10.6%至+7.8%以内。在35份氯胺酮阳性样本中的19份样本中，脱氢去甲氯胺酮的浓度高于氯胺酮或去甲氯胺酮。使用新方法分析了一组先前测定含有至少一种目标分析物的110份人类尿液样本，结果与先前结果高度一致。
