Zhang Xiao, Zheng Ying-Jie, Wang Fa-Di, Gao Mei-Yang, Zhu Jian-Fu, Jiang Qing-Wu
Department of Epidemiology, School of Public Health, Central South University, Changsha 410078, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2005 Dec;26(12):984-7.
To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.
Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced.
13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA. Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3% - 85.3%, 76.0% - 80.0% and 84.7% - 95.3% nucleotide sequence identity with HEV genotype I, II, III and IV respectively in the region (nt6317- 6466).
HEV circulating in humans and swine in the area belonged to genotype IV.
研究中国南方农村地区从人分离出的猪的基因型及其与戊型肝炎病毒（HEV）的关系。
采用ORF2引物的RT-nPCR法对从戊型肝炎病毒IgM阳性正常人、急性戊型肝炎患者以及同一地区猪采集的标本进行HEV RNA检测。对阳性PCR产物进行克隆和测序。
132份猪粪便样本中有13份、26份正常人戊型肝炎病毒IgM阳性血清中有4份、4份急性戊型肝炎患者粪便样本和血清中有1份的HEV RNA检测呈阳性。序列分析数据显示，在10株分离株中，核苷酸水平的同一性为89.3%-100.0%，在该区域（nt6317-6466）与戊型肝炎病毒基因型I、II、III和IV的核苷酸序列同一性分别为78.7%-84.7%、83.3%-85.3%、76.0%-80.0%和84.7%-95.3%。
该地区人和猪中传播的戊型肝炎病毒属于基因型IV。
