[抗戊型肝炎病毒IgG检测及部分戊型肝炎病毒RNA序列分析]
[Detection of anti-HEV IgG and analysis of partial HEV RNA sequence].
作者信息
Zhu Yong-hong, Zhuang Hui, Dong Qing-ming, Chen Yan-feng, Li Zheng-tai, Wu Hua, Liu Jian
机构信息
Department of Microbiology and Parasitology, Health Science Center, Peking University, Beijing 100083, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2003 Jul;11(7):405-7.
OBJECTIVES
To investigate HEV infection in swine and the genotype relationship between swine and human HEV.
METHODS
Anti-HEV IgG antibody was detected in the sera of swine using enzyme linked immunoassay (EIA), and HEV RNA was amplified by reverse transcription nested polymerase chain reaction (RT-nPCR). The Vector NTI Suite 7 and TreeView softwares were used for nucleotide sequences phylogenetic analysis of HEV isolated from human and swine.
RESULTS
The anti-HEV IgG positive rate was 16.67% (18/108). Among the 18 anti-HEV IgG positive sera, 2 sequences (11.11%, called S18 and S43, respectively) of HEV ORF1 (102-387bp) were amplified, with the identity of 99% between them. They had 76% to 77%, 78%, 76% to 79%, 85% to 86%, 77%, 80%, 79% and 75% - 79% homology at the nucleotide level with human HEV genotypes 1 to 8, respectively. One (S18) of them was also amplified out in ORF2 region (5,994-6 297bp) and showed 76% to 78%, 74%, 74% to 77%, and 85% to 94% identity with human HEV genotypes 1 to 4 at the nucleotide level, respectively.
CONCLUSION
HEV sequences isolated from swine belong to human HEV genotype 4.
目的
调查猪戊型肝炎病毒（HEV）感染情况以及猪和人HEV的基因型关系。
方法
采用酶联免疫吸附测定（EIA）检测猪血清中的抗HEV IgG抗体，并用逆转录巢式聚合酶链反应（RT - nPCR）扩增HEV RNA。使用Vector NTI Suite 7和TreeView软件对从人和猪分离的HEV核苷酸序列进行系统发育分析。
结果
抗HEV IgG阳性率为16.67%（18/108）。在18份抗HEV IgG阳性血清中，扩增出2条HEV开放阅读框1（ORF1，102 - 387bp）序列（分别称为S18和S43，占11.11%），它们之间的同一性为99%。它们在核苷酸水平上与人HEV基因型1至8的同源性分别为76%至77%、78%、76%至79%、85%至86%、77%、80%、79%和75% - 79%。其中一条序列（S18）在ORF2区域（5,994 - 6 297bp）也被扩增出来，在核苷酸水平上与人类HEV基因型1至4的同一性分别为76%至78%、74%、74%至77%和85%至94%。
结论
从猪分离的HEV序列属于人类HEV基因型4。
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