用于DNA标记的新型铕（III）发光螯合物。
New luminescent europium(III) chelates for DNA labeling.
作者信息
Nishioka Takuya, Yuan Jingli, Yamamoto Yuji, Sumitomo Keiko, Wang Zhen, Hashino Kimikazu, Hosoya Chihiro, Ikawa Keisuke, Wang Guilan, Matsumoto Kazuko
机构信息
Department of Chemistry, Advanced Research Institute for Science and Engineering, Waseda University, Shinjuku-ku 169-8555, Tokyo, Japan.
出版信息
Inorg Chem. 2006 May 15;45(10):4088-96. doi: 10.1021/ic051276g.
The new europium(III) chelate [2,2',2'',2'''-[[4'-(aminobiphenyl-4-yl)-2,2':6',2''-terpyridine- 6,6''-diyl]bis(methylenenitrilo)]tetrakis(acetato)] europium(III) (ATBTA-Eu3+) and its 4,6-dichloro-1,3,5-triazinyl and succinimidyl derivatives (DTBTA and NHS-ATBTA, respectively) were synthesized and characterized. Both labeling complexes DTBTA-Eu3+ and NHS-ATBTA-Eu3+ are luminescent. Especially DTBTA-Eu3+ is strongly luminescent, with a luminescence quantum yield of 9.1%, molar extinction coefficient of 3.1 x 10(4) cm(-1) M(-1) (335 nm), and luminescence lifetime of 1.02 ms. The excitation and emission maximum wavelengths of DTBTA-Eu3+ are 335 and 616 nm, respectively. The complex is very stable in aqueous buffers, with a conditional formation constant log K(DTBTA-Eu) of 25.0 at pH 8, and can be conjugated to DNA and proteins. The chelates are also highly resistant to thermal decomposition, photodegradation, and ozone oxidation. These properties prove that DTBTA-Eu3+ is suitable as a luminescence label in DNA assays.
新型铕（III）螯合物[2,2',2'',2'''-[[4'-(氨基联苯-4-基)-2,2':6',2''-三联吡啶-6,6''-二基]双(亚甲基腈基)]四(乙酸根)]铕（III）（ATBTA-Eu3+）及其4,6-二氯-1,3,5-三嗪基和琥珀酰亚胺基衍生物（分别为DTBTA和NHS-ATBTA）被合成并表征。两种标记配合物DTBTA-Eu3+和NHS-ATBTA-Eu3+均具有发光性。特别是DTBTA-Eu3+具有强烈的发光性，其发光量子产率为9.1%，摩尔消光系数为3.1×10(4) cm(-1) M(-1)（335 nm），发光寿命为1.02 ms。DTBTA-Eu3+的激发和发射最大波长分别为335和616 nm。该配合物在水性缓冲液中非常稳定，在pH 8时条件形成常数log K(DTBTA-Eu)为25.0，并且可以与DNA和蛋白质共轭。这些螯合物还对热分解、光降解和臭氧氧化具有高度抗性。这些性质证明DTBTA-Eu3+适合作为DNA分析中的发光标记物。
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