Department of Instrumentation and Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Chemistry. 2010 Jun 11;16(22):6464-72. doi: 10.1002/chem.201000528.
A lanthanide-complex-based ratiometric luminescence probe specific for peroxynitrite (ONOO(-)), 4'-(2,4-dimethoxyphenyl)-2,2':6',2''-terpyridine-6,6''-diyl]bis(methylenenitrilo)tetrakis(acetate)-Eu(3+)/Tb(3+) ([Eu(3+)/Tb(3+)(DTTA)]), has been designed and synthesized. Both [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] are highly water soluble with large stability constants at approximately 10(20), and strongly luminescent with luminescence quantum yields of 10.0 and 9.9%, respectively, and long luminescence lifetimes of 1.38 and 0.26 ms, respectively. It was found that the luminescence of [Tb(3+)(DTTA)] could be quenched by ONOO(-) rapidly and specifically in aqueous buffers, while that of [Eu(3+)(DTTA)] did not respond to the addition of ONOO(-). Thus, by simply mixing [Eu(3+)(DTTA)] and [Tb(3+)(DTTA)] in an aqueous buffer, a ratiometric luminescence probe specific for time-gated luminescence detection of ONOO(-) was obtained. The performance of [Tb(3+)(DTTA)] and [Eu(3+)/Tb(3+)(DTTA)] as the probes for luminescence imaging detection of ONOO(-) in living cells was investigated. The results demonstrated the efficacy and advantages of the new ratiometric luminescence probe for highly sensitive luminescence bioimaging application.
一种基于镧系元素配合物的用于过氧亚硝酸根（ONOO(-)）的比率型荧光探针，4'-(2,4-二甲氧基苯基)-2,2':6',2''-三联吡啶-6,6''-二基]双(亚甲基亚硝酰基)四乙酸-铕(3+)/铽(3+) ([Eu(3+)/Tb(3+)(DTTA)]），已被设计和合成。[Eu(3+)(DTTA)]和[Tb(3+)(DTTA)]均具有高水溶性和约 10(20) 的大稳定常数，并且分别具有 10.0%和 9.9%的强荧光量子产率和分别为 1.38 和 0.26 ms 的长荧光寿命。研究发现，在水缓冲液中，[Tb(3+)(DTTA)]的荧光可以被 ONOO(-)快速且特异性地猝灭，而[Eu(3+)(DTTA)]的荧光则不会对 ONOO(-)的加入产生响应。因此，通过简单地将[Eu(3+)(DTTA)]和[Tb(3+)(DTTA)]混合在水缓冲液中，得到了一种用于时间门控检测 ONOO(-)的比率型荧光探针。还研究了[Tb(3+)(DTTA)]和[Eu(3+)/Tb(3+)(DTTA)]作为活细胞中 ONOO(-)的荧光成像检测探针的性能。结果表明，新型比率型荧光探针对于高灵敏度的荧光生物成像应用具有良好的效果和优势。
