Ross Eric E, Joubert James R, Wysocki Ronald J, Nebesny Ken, Spratt Tony, O'Brien David F, Saavedra S Scott
University of Arizona, Department of Chemistry, Tucson, Arizona 85721, USA.
Biomacromolecules. 2006 May;7(5):1393-8. doi: 10.1021/bm050727l.
The use of polymerized lipid bilayers as substrates for microcontact printing (muCP) of protein films was investigated. We have previously shown that vesicle fusion of bis-SorbPC, a dienoate lipid, on glass and silica substrates, followed by redox-initiated radical polymerization, produces a planar supported lipid bilayer (PSLB) that is ultrastable(1a) [Ross, E. E.; Rozanski, L. J.; Spratt, T.; Liu, S.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 1752] and highly resistant to nonspecific adsorption of dissolved proteins [Ross, E. E.; Spratt, T.; Liu, S.; Rozanski, L. J.; O'Brien, D. F.; Saavedra, S. S. Langmuir 2003, 19, 1766].(1b) Here we demonstrate that muCP of bovine serum albumin (BSA) onto a dried poly(bis-SorbPC) PSLB from a poly(dimethylsiloxane) (PDMS) stamp produces a layer of strongly adsorbed protein, comparable in surface coverage to films printed on glass surfaces. Immobilization of proteins on poly(PSLB)s has potential applications in biosensing, and this work shows that direct muCP of proteins is a technically simple approach to create immobilized monolayers, as well as multilayers of different proteins.
研究了使用聚合脂质双层作为蛋白质膜微接触印刷（μCP）的底物。我们之前已经表明，双烯酸酯脂质双-SorbPC在玻璃和二氧化硅底物上进行囊泡融合，然后通过氧化还原引发的自由基聚合，可产生超稳定的平面支撑脂质双层（PSLB）（1a）[罗斯，E.E.；罗赞斯基，L.J.；斯普拉特，T.；刘，S.；奥布赖恩，D.F.；萨韦德拉，S.S.《朗缪尔》2003年，19卷，1752页]，并且对溶解蛋白质的非特异性吸附具有高度抗性[罗斯，E.E.；斯普拉特，T.；刘，S.；罗赞斯基，L.J.；奥布赖恩，D.F.；萨韦德拉，S.S.《朗缪尔》2003年，19卷，1766页]。（1b）在此我们证明，使用聚二甲基硅氧烷（PDMS）印章将牛血清白蛋白（BSA）微接触印刷到干燥的聚（双-SorbPC）PSLB上会产生一层强烈吸附的蛋白质，其表面覆盖率与印刷在玻璃表面的膜相当。将蛋白质固定在聚（PSLB）上在生物传感方面具有潜在应用，并且这项工作表明蛋白质的直接微接触印刷是一种技术上简单的方法，可用于创建固定化单层以及不同蛋白质的多层。
