Ozawa T, Hanaki A
Division of Chemical Pharmacology, National Institute of Radiological Sciences, Chiba.
Biochem Int. 1991 Nov;25(4):783-8.
Formation of superoxide ion (O2-) from the reaction of CuII(en)2 (en: ethylenediamine) with hydrogen peroxide (H2O2) was first determined spectrophotometrically by use of nitro blue tetrazolium (NBT) in aqueous solutions. From this result, it has been suggested that superoxide ion is generated as an intermediate at the first reaction step between CuII(en)2 and H2O2.
通过使用硝基蓝四氮唑(NBT)在水溶液中进行分光光度法首次测定了CuII(乙二胺)(en:乙二胺)与过氧化氢(H2O2)反应生成超氧离子(O2-)。根据该结果,有人提出超氧离子是在CuII(乙二胺)和H2O2之间的第一步反应中作为中间体生成的。