Van den Hove D L A, Lauder J M, Scheepens A, Prickaerts J, Blanco C E, Steinbusch H W M
Department of Pediatrics, Research Institute Growth and Development (GROW), Faculty of Medicine, Maastricht University, 6202 AZ Maastricht, The Netherlands.
Brain Res. 2006 May 23;1090(1):29-34. doi: 10.1016/j.brainres.2006.03.057. Epub 2006 May 3.
Exposure of a pregnant woman to physical and/or psychological stress might affect her offspring by promoting the development of various learning, behavioral and/or mood disorders in later life. The 5-HT1A and 5-HT2A receptors are prominently implicated in the modulation of anxiety and mood-related behaviors. Using a semi-quantitative radiolabel immunocytochemical analysis (immunobinding), we studied the effect of prenatal stress on binding of these two receptor subtypes in the hippocampus of 4-week-old male and female Fischer 344 rats. Levels of 5-HT1A immunobinding in the ventral hippocampus, which is primarily implicated in emotional processing, were significantly decreased in male offspring after prenatal stress. A trend towards a decrease was observed in the ventral hippocampus of females. In contrast, 5-HT1A immunobinding within the dorsal hippocampus, which is mainly related to learning and memory, was not affected by prenatal stress in offspring of either gender. Likewise, no significant differences between control and prenatally stressed rats were observed for levels of 5-HT2A immunobinding in either part of the hippocampus or gender. The observed reduction in hippocampal 5-HT1A receptor binding in male offspring after prenatal stress may have important consequences for adult anxiety- and depressive-like behavior.
孕妇遭受身体和/或心理压力可能会影响其后代，导致后代在日后生活中出现各种学习、行为和/或情绪障碍。5-HT1A和5-HT2A受体在调节焦虑和情绪相关行为中起重要作用。我们使用半定量放射性标记免疫细胞化学分析（免疫结合法），研究了产前应激对4周龄雄性和雌性Fischer 344大鼠海马体中这两种受体亚型结合的影响。主要参与情绪处理的腹侧海马体中，产前应激后雄性后代的5-HT1A免疫结合水平显著降低。在雌性腹侧海马体中观察到有下降趋势。相比之下，主要与学习和记忆相关的背侧海马体中的5-HT1A免疫结合不受产前应激对任何性别的后代的影响。同样，在海马体的任何部位或性别中，对照大鼠和产前应激大鼠的5-HT2A免疫结合水平均未观察到显著差异。产前应激后雄性后代海马体中5-HT1A受体结合的减少可能对成年后的焦虑样和抑郁样行为产生重要影响。
