Solomon A, De Fanti B A, Martínez J A
Department of Physiology and Nutrition, University of Navarra, C/Irunlarrea, 1, 31008, Pamplona, Spain.
Neuropeptides. 2006 Jun;40(3):169-75. doi: 10.1016/j.npep.2006.03.003. Epub 2006 May 4.
Three extrahypothalamic areas, the nucleus of the tractus solitari (NTS), the central nucleus of the amygdala (CeA) and the dorsal raphe nucleus (DRN), all potentially involved in peripheral ghrelin signalling of appetite control mediated by the glucose levels were examined. Thus, a specific anti-ghrelin antibody (AGA) was intravenously administered in order to remove the ghrelin signalling and then, subsequently, 4-h food intake, plasma glucose levels and the brain c-fos expression in the NTS, CeA and DRN were assessed. Food intake significantly decreased when the AGA was administered. NTS c-Fos expression was significantly augmented by insulin, while it was significantly decreased by the AGA. These results suggest that the NTS is a part of the ghrelin pathway that regulates the orexigenic signalling cascade, which may be triggered by a drop in blood glucose levels mediated by insulin.
研究了三个下丘脑外区域，即孤束核（NTS）、杏仁核中央核（CeA）和中缝背核（DRN），它们都可能参与由葡萄糖水平介导的食欲控制的外周胃饥饿素信号传导。因此，静脉注射一种特异性抗胃饥饿素抗体（AGA）以消除胃饥饿素信号，随后评估4小时食物摄入量、血浆葡萄糖水平以及NTS、CeA和DRN中的脑c-fos表达。注射AGA后食物摄入量显著减少。胰岛素显著增加NTS的c-Fos表达，而AGA则使其显著降低。这些结果表明，NTS是调节食欲信号级联反应的胃饥饿素途径的一部分，该途径可能由胰岛素介导的血糖水平下降触发。
