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超高帧率回顾性超声显微成像及小鼠体内血流可视化

Ultrahigh frame rate retrospective ultrasound microimaging and blood flow visualization in mice in vivo.

作者信息

Chérin Emmanuel, Williams Ross, Needles Andrew, Liu Godwin, White Christopher, Brown Allison S, Zhou Yu-Qing, Foster F Stuart

机构信息

Sunnybrook and Women's College Health Sciences Centre, Toronto, Ontario, Canada.

出版信息

Ultrasound Med Biol. 2006 May;32(5):683-91. doi: 10.1016/j.ultrasmedbio.2005.12.015.

DOI:10.1016/j.ultrasmedbio.2005.12.015
PMID:16677928
Abstract

To overcome frame rate limitations in high-frequency ultrasound microimaging, new data acquisition techniques have been implemented for 2-D (B-scan) and color flow visualization. These techniques, referred to as retrospective B-scan imaging (RBI) and retrospective color flow imaging (RCFI) are based on the use of the electrocardiogram (ECG) to trigger signal acquisitions. B-scan and color flow images are reconstructed by retrospectively assembling the processed data on a line-by-line basis. Retrospective techniques are used to produce the first in vivo B-scan and color flow images of mouse carotid arteries at frame rates up to 10,000 fps. Retrospective B-scan images of mouse heart were also produced at frame rates of 1000 fps using a version of RBI implemented on a commercial imaging system (Vevo660, VisualSonics, Toronto, ON, Canada). This technology enables detailed in vivo biomechanical studies of dynamic tissues such as the myocardium of the mouse heart with high temporal resolution.

摘要

为克服高频超声显微成像中的帧率限制,已针对二维(B 扫描)和彩色血流可视化实施了新的数据采集技术。这些技术被称为回顾性 B 扫描成像(RBI)和回顾性彩色血流成像(RCFI),它们基于利用心电图(ECG)触发信号采集。B 扫描和彩色血流图像通过逐行回顾性地组装处理后的数据来重建。回顾性技术用于以高达 10000 帧/秒的帧率生成小鼠颈动脉的首张体内 B 扫描和彩色血流图像。使用在商业成像系统(Vevo660,VisualSonics,多伦多,安大略省,加拿大)上实施的 RBI 版本,还以 1000 帧/秒的帧率生成了小鼠心脏的回顾性 B 扫描图像。这项技术能够以高时间分辨率对诸如小鼠心脏心肌等动态组织进行详细的体内生物力学研究。

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